To improve the efficiency of polysaccharide extraction, vacuum technology was used to breakdown the cell wall to extract polysaccharides from Lentinus edodes stipe, and the optimal parameters were optimized by response surface methodology. Then the antioxidant activities in vitro of the polysaccharides were evaluated. Results showed that the optimal conditions for polysaccharide extraction were temperature of 62 °C, vacuum of 0.08 MPa, stirring speed of 1200 r/min, time of 25 min and material/liquid ratio of 1:26 (g/mL). Under these conditions, the yield of polysaccharide was 4.28%. The decolorization rate and deproteinization rate of polysaccharides treated by kaolin and hydrogen peroxide were higher than that of activated carbon. However, there was no significant influence on the polysaccharide retention rate, that was about 80%. Results also indicated that polysaccharide treated with activated carbon could obtain higher antioxidant activity.

中文翻译：

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香菇柄真空条件下多糖的提取及其体外抗氧化活性。

为了提高多糖的提取效率，采用真空技术对细胞壁进行分解，从香菇柄中提取多糖，并通过响应面法对最佳参数进行了优化。然后评估了多糖的体外抗氧化活性。结果表明，提取多糖的最佳条件为温度62°C，真空度0.08 MPa，搅拌速度1200 r / min，时间25 min，料液比1:26（g / mL）。在这些条件下，多糖的产率为4.28％。高岭土和过氧化氢处理的多糖的脱色率和脱蛋白率均高于活性炭。但是，对多糖的保留率没有显着影响，约为80％。