The zebrafish is a model organism that allows in vivo studies to be performed at a scale usually restricted to in vitro studies. As such, the zebrafish is well suited to in vivo screens, in which thousands of small molecules are tested for their ability to modify disease phenotypes in zebrafish disease models. Numerous approaches have been developed for modeling human diseases in zebrafish, including mutagenesis, transgenesis, pharmacological approaches, wounding, and exposure to infectious or cancerous agents. We review the various strategies for modeling human diseases in zebrafish and discuss important considerations when developing zebrafish models for use in in vivo small molecule screens.

中文翻译：

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为体内小分子筛查开发斑马鱼疾病模型。

斑马鱼是一种模式生物，可以以通常限于体外研究的规模进行体内研究。这样，斑马鱼非常适合于体内筛选，其中测试了数千个小分子修饰斑马鱼疾病模型中疾病表型的能力。已经开发出许多方法来模拟斑马鱼中的人类疾病，包括诱变，转基因，药理学方法，伤口以及暴露于传染性或癌性。我们回顾了在斑马鱼中模拟人类疾病的各种策略，并讨论了开发用于体内小分子筛选的斑马鱼模型时的重要考虑因素。