Eugenia uniflora is an Atlantic Forest native species, occurring in contrasting edaphoclimatic environments. The identification of genes involved in response to abiotic factors is very relevant to help in understanding the processes of local adaptation. 1-Pyrroline-5-carboxylate synthetase (P5CS) is one interesting gene to study in this species since it encodes a key enzyme of proline biosynthesis, which is an osmoprotectant during abiotic stress. Applying in silico analysis, we identified one P5CS gene sequence of E. uniflora (EuniP5CS). Phylogenetic analysis, as well as, gene and protein structure investigation, revealed that EuniP5CS is a member of P5CS gene family. Plants of E. uniflora from two distinct environments (restinga and riparian forest) presented differences in the proline accumulation and P5CS expression levels under growth-controlled conditions. Both proline accumulation and gene expression level of EuniP5CS were higher in the genotypes from riparian forest than those from restinga. When these plants were submitted to drought stress, EuniP5CS gene was up-regulated in the plants from restinga, but not in those from riparian forest. These results demonstrated that EuniP5CS is involved in proline biosynthesis in this species and suggest that P5CS gene may be an interesting candidate gene in future studies to understand the processes of local adaptation in E. uniflora.

中文翻译：

---

大西洋森林原生种Eugenia uniflora L的两个不同种群中P5CS（Δ1-吡咯啉-5-羧酸合酶）基因的表征和表达分析。

紫丁香（Eugenia uniflora）是大西洋森林的原生物种，在相反的水生气候环境中出现。鉴定对非生物因子应答的基因非常有助于理解局部适应过程。1-吡咯啉-5-羧酸酯合成酶（P5CS）是在该物种中研究的一个有趣的基因，因为它编码脯氨酸生物合成的关键酶，该酶是非生物胁迫期间的渗透保护剂。应用计算机分析，我们确定了一种E. uniflora（EuniP5CS）P5CS基因序列。系统发育分析以及基因和蛋白质的结构研究表明，EuniP5CS是P5CS基因家族的成员。E.的植物 来自两个不同环境（restinga和河岸森林）的单花植物在生长受控条件下的脯氨酸积累和P5CS表达水平存在差异。沿岸森林的基因型中，EuniP5CS的脯氨酸积累和基因表达水平均高于resta的基因型。当这些植物遭受干旱胁迫时，来自restasta的植物中的EuniP5CS基因被上调，而来自河岸林的植物中的EuniP5CS基因却被上调。这些结果表明，EuniP5CS参与了该物种的脯氨酸生物合成，并表明P5CS基因可能是未来研究中了解单花大肠杆菌局部适应过程的有趣候选基因。沿岸森林的基因型中，EuniP5CS的脯氨酸积累和基因表达水平均高于resta的基因型。当这些植物遭受干旱胁迫时，来自restasta的植物中的EuniP5CS基因被上调，而来自河岸林的植物中的EuniP5CS基因却被上调。这些结果表明，EuniP5CS参与了该物种的脯氨酸生物合成，并表明P5CS基因可能是未来研究中了解单花大肠杆菌局部适应过程的有趣候选基因。沿岸森林的基因型中，EuniP5CS的脯氨酸积累和基因表达水平均高于resta的基因型。当这些植物遭受干旱胁迫时，来自restasta的植物中的EuniP5CS基因被上调，而来自河岸林的植物中的EuniP5CS基因却被上调。这些结果表明，EuniP5CS参与了该物种的脯氨酸生物合成，并表明P5CS基因可能是未来研究中了解单花大肠杆菌局部适应过程的有趣候选基因。