Cryopreservation of genetic material can become an important tool for user groups in imperiled fishes, wild fisheries, aquaculture, and biomedical research. Persistent challenges within aquatic species cryopreservation are standardization and reliable collection of diverse, high quality samples. The overall goal of this study was to work with different user groups and cryopreserve sperm on-site at their facilities to evaluate the uses and challenges of a mobile laboratory with high-throughput and quality control capabilities comparable to those of a specialized centralized facility. The objectives were to demonstrate collection and cryopreservation of sperm of: 1) large-bodied freshwater Blue Catfish (Ictalurus furcatus) for aquaculture; 2) small-bodied freshwater Xiphophorus for biomedical and imperiled repository development, and 3) saltwater Red Snapper (Lutjanus campechanus) for wild fisheries research. Over the course of this project, the mobile laboratory traveled more than 4,000 km collecting germplasm from more than 650 male fishes. A total of 136 Blue Catfish were processed in 2015 and 2016 resulting in a total of 6,146 0.5-mL French straws. A total of 521 males from 11 different species in the genus Xiphophorus were processed over 4 d in 2015 resulting in a total of 488 0.25-mL French straws. And, a total of 17 Red Snapper males were processed during 2015 resulting in a total of 316 0.5-mL French straws. This is the first development of a mobile laboratory with high-throughput capability for aquatic species. User groups would no longer be limited to germplasm resources that can only be shipped as samples or transported as live animals to a central cryopreservation facility. Mobile laboratories create opportunities to collect higher quality germplasm, provide access to new species, and enable direct cooperation, including training, with a wide variety of user groups and applications.

中文翻译：

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水生物种冷冻保存移动实验室的现场能力

遗传物质的冷冻保存可以成为濒危鱼类、野生渔业、水产养殖和生物医学研究用户群体的重要工具。水生物种冷冻保存中持续存在的挑战是标准化和可靠地收集多样化的高质量样品。这项研究的总体目标是与不同的用户群体合作，并在其设施现场冷冻保存精子，以评估具有与专门的集中设施相当的高通量和质量控制能力的移动实验室的用途和挑战。目的是演示以下精子的采集和冷冻保存： 1) 用于水产养殖的大型淡水蓝鲶鱼（Ictalurusfurcatus）；2) 用于生物医学和濒危资源库开发的小型淡水剑鱼，以及 3) 用于野生渔业研究的咸水红鲷鱼 (Lutjanus Campechanus)。在该项目过程中，移动实验室行驶了 4,000 多公里，从 650 多条雄性鱼类中收集了种质。2015 年和 2016 年总共加工了 136 条蓝鲶鱼，最终获得了 6,146 根 0.5 毫升法国吸管。2015 年，来自剑尾属 11 个不同物种的总共 521 只雄性剑尾鱼经过 4 天的处理，总共产生了 488 根 0.25 毫升的法国吸管。2015 年，总共加工了 17 只雄性红鲷鱼，最终获得了 316 根 0.5 毫升法国吸管。这是第一个针对水生物种开发的具有高通量能力的移动实验室。用户群体将不再局限于只能作为样本运输或作为活体动物运输到中央低温保存设施的种质资源。移动实验室创造了收集更高质量种质的机会，提供新物种的获取，并实现与各种用户群体和应用程序的直接合作（包括培训）。