Objective: To synthesise HSA-RB-DOX nanoparticles, measure its characteristics and preliminarily evaluate its anti-cancer effects.

Methods: Doxorubicin (DOX) and Rose Bengal (RB) were co-delivered using albumin as a carrier. HSA-RB-DOX nanoparticles were prepared by RB-induced self-assembly of albumin. Its characteristics were measured and anti-cancer effects were tested in MCF-7 cells and tumour-bearing mice.

Results: HSA-RB-DOX nanoparticle with a mean size of 42 nm was stable in different medium and behaved controlled release characteristic. It was well took in MCF-7 cells and inhibited MCF-7 cells proliferation by inducing reactive oxygen species (ROS) production. It retained a much higher blood concentration up to 12 h and accumulated more in tumour tissues. In tumour-bearing mice, HSA-RB-DOX nanoparticles inhibited tumour growth and even decreased its volume from 100 to 50 mm³, with barely no influence on body weight.

Conclusions: HSA-RB-DOX nanoparticles may be potentially used for enhanced treatment of breast cancer.

目的：合成HSA-RB-DOX纳米粒子，测量其特性并初步评价其抗癌作用。

方法：以白蛋白为载体，共同递送阿霉素（DOX）和玫瑰红（RB）。HSA-RB-DOX纳米粒子是通过RB诱导的白蛋白自组装制备的。在MCF-7细胞和荷瘤小鼠中测量了其特征并测试了抗癌作用。

结果：平均粒径为42 nm的HSA-RB-DOX纳米颗粒在不同介质中稳定，并具有控释特性。它可以很好地吸收MCF-7细胞，并通过诱导活性氧（ROS）产生来抑制MCF-7细胞的增殖。长达12小时，它保留了更高的血液浓度，并在肿瘤组织中积累了更多。在荷瘤小鼠中，HSA-RB-DOX纳米颗粒可抑制肿瘤生长，甚至将其体积从100 mm ³减少至50 mm ³，而对体重几乎没有影响。

结论： HSA-RB-DOX纳米粒子可能潜在地用于增强乳腺癌的治疗。