Asbestos fiber has been associated with mesothelioma and lung cancer. However, the carcinogenic risks of other fiber nanomaterials with morphological similarities to asbestos have not been fully studied. Ultra-long silver nanowires (AgNWs) are increasingly used fiber-shaped nanomaterials with a high aspect ratio, but very few studies have investigated their health risks. Here, proliferation abnormalities of lung epithelial cells induced by ultra-long AgNWs were investigated. Ultra-long AgNW treatment induced dose- and diameter-dependent increase in the ratio of multinucleated cells. Further, proteins involved in mitosis and cytokinesis, including Aurora A, p-Histone 3 (ser10), RhoA, p-MLC, and myosin IIb, were significantly upregulated after an ultra-long AgNW treatment, leading to mitotic abnormalities and cytokinetic failure. Meanwhile, exposure to ultra-long AgNWs induced cell cycle arrest. Interestingly, a series of experiments demonstrated that ROS generation and Ag+ release were not responsible for the multinucleation induced by ultra-long AgNWs, but ultra-long AgNWs in the intercellular bridge might obstruct the contractile ring and inhibit abscission of the cytokinetic furrow by direct physical contact. Altogether, our findings indicate that ultra-long AgNWs can induce chromosomal instability, which has important consequences for the safety of ultra-long AgNWs to human health.

中文翻译：

---

超长银纳米线在A549细胞中诱导有丝分裂异常和细胞动力学衰竭。

石棉纤维与间皮瘤和肺癌有关。但是，尚未充分研究形态与石棉相似的其他纤维纳米材料的致癌风险。超长银纳米线（AgNWs）越来越多地用于具有高长宽比的纤维状纳米材料，但是很少有研究调查其健康风险。在这里，研究了由超长AgNWs引起的肺上皮细胞的增殖异常。超长AgNW处理可引起剂量和直径依赖性多核细胞比例的增加。此外，超长的AgNW处理后，涉及有丝分裂和胞质分裂的蛋白，包括Aurora A，p-Histone 3（ser10），RhoA，p-MLC和肌球蛋白IIb，被显着上调，导致有丝分裂异常和细胞动力学衰竭。与此同时，暴露于超长AgNWs诱导的细胞周期停滞。有趣的是，一系列实验表明ROS的产生和Ag +的释放与超长AgNWs诱导的多核化无关，但是细胞间桥中的超长AgNWs可能会通过直接物理方式阻塞收缩环并抑制细胞动力学犁沟的脱落。联系。总之，我们的发现表明，超长AgNW可以诱发染色体不稳定性，这对于超长AgNW对人类健康的安全性具有重要影响。但细胞间桥中的超长AgNW可能会通过直接物理接触而阻塞收缩环并抑制细胞动力学沟的脱落。总之，我们的发现表明，超长AgNW可以诱发染色体不稳定性，这对于超长AgNW对人类健康的安全性具有重要影响。但细胞间桥中的超长AgNW可能会通过直接物理接触而阻塞收缩环并抑制细胞动力学沟的脱落。总之，我们的发现表明，超长AgNW可以诱发染色体不稳定性，这对于超长AgNW对人类健康的安全性具有重要影响。