The distinct combination of homing receptors such as selectins, chemokine receptors, and integrins directs the migration of lymphocytes throughout the body. Upon activation lymphocytes irreversibly switch their set of homing receptors, now guiding them to entirely different destinations. Here we report that exposure of naive B cells to the microenvironment of the peritoneal cavity modulates their migration propensities in the absence of antigenic stimulation. B1 and B2 cells isolated from the peritoneal cavity reenter this compartment more efficiently compared with splenic follicular B cells. Moreover, when kept in the peritoneal cavity splenic follicular B cells gain such increased capability to reenter this compartment. These altered migratory capacities are reflected by an up-regulation of the chemokine receptors CXCR4 and CXCR5 and beta7 integrin by the peritoneum-experienced splenic B cells, among which CXCR5 is instrumental in directing B cells into the peritoneal cavity. Moreover, intraperitoneal transfer of plasma blasts favors their migration into the small intestine presumably before class switch recombination occurs, demonstrating that a reconfigured transient migration pattern is not restricted to naive cells. In conclusion, these data demonstrate a hitherto unrecognized role for tissue-specific cues, altering the migratory capacity of B1, naive B2, as well as antigen-experienced B2 cells.

中文翻译：

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腹膜微环境使 B 细胞进入体腔和小肠

归巢受体（如选择素、趋化因子受体和整联蛋白）的独特组合指导淋巴细胞在全身的迁移。激活后，淋巴细胞会不可逆地切换它们的归巢受体组，现在将它们引导到完全不同的目的地。在这里我们报告幼稚 B 细胞暴露于腹腔微环境会在没有抗原刺激的情况下调节它们的迁移倾向。与脾滤泡 B 细胞相比，从腹腔分离的 B1 和 B2 细胞更有效地重新进入该隔室。此外，当保存在腹腔中时，脾滤泡 B 细胞重新进入该隔室的能力增强。这些改变的迁移能力反映在腹膜经历过的脾 B 细胞对趋化因子受体 CXCR4 和 CXCR5 和 beta7 整合素的上调，其中 CXCR5 有助于将 B 细胞引导到腹腔。此外，浆母细胞的腹膜内转移有利于它们在类别转换重组发生之前迁移到小肠，这表明重新配置的瞬时迁移模式不限于幼稚细胞。总之，这些数据证明了组织特异性线索迄今为止未被认识到的作用，改变了 B1、幼稚 B2 以及抗原经历过的 B2 细胞的迁移能力。浆母细胞的腹膜内转移有利于它们在类别转换重组发生之前迁移到小肠，这表明重新配置的瞬时迁移模式不仅限于幼稚细胞。总之，这些数据证明了组织特异性线索迄今为止未被认识到的作用，改变了 B1、幼稚 B2 以及抗原经历过的 B2 细胞的迁移能力。浆母细胞的腹膜内转移有利于它们在类别转换重组发生之前迁移到小肠，这表明重新配置的瞬时迁移模式不仅限于幼稚细胞。总之，这些数据证明了组织特异性线索的迄今为止未被认识的作用，改变了 B1、幼稚 B2 以及抗原经历过的 B2 细胞的迁移能力。