Autoreactive B cells that appear to be inactivated can be found in healthy individuals. In this study, we examined the potential of these anergic cells to become activated. We show that anergy of anti-double-stranded DNA (dsDNA) B cells in BALB/c mice is readily reversed, requiring only the provision of T cell help. We further show that spontaneous loss of anergy among anti-dsDNA B cells in autoimmune lpr/lpr mice occurs in two phases: an abortive initial response to T help followed by full loss of tolerance. Strikingly, the abortive response can be reproduced in nonautoimmune mice when CD4+CD25+ T regulatory cells are administered in conjunction with CD4+ T helper cells, suggesting that loss of B cell tolerance may require both the production of T cell help and the overcoming of T suppression.

中文翻译：

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T辅助细胞和T调节细胞对抗双链DNA B细胞调节的影响。

在健康个体中可以发现似乎已失活的自身反应性B细胞。在这项研究中，我们检查了这些无能细胞被激活的可能性。我们显示，BALB / c小鼠中的抗双链DNA（dsDNA）B细胞无反应性很容易被逆转，只需要提供T细胞帮助即可。我们进一步表明，在自身免疫的lpr / lpr小鼠中，抗dsDNA B细胞之间的自发性丧失自发分两个阶段发生：对T help的流产初期反应，随后完全丧失了耐受性。引人注目的是，当将CD4 + CD25 + T调节细胞与CD4 + T辅助细胞一起给药时，非自身免疫小鼠中可产生流产反应，这表明B细胞耐受性的丧失可能既需要产生T细胞，又需要克服T抑制。 。