Conceptus development and elongation is required for successful pregnancy establishment in ruminants and is coincident with the production of interferon τ (IFNT) and prostaglandins (PGs). In both the conceptus trophectoderm and endometrium, PGs are primarily synthesized through a prostaglandin-endoperoxide synthase 2 (PTGS2) pathway and modify endometrial gene expression and thus histotroph composition in the uterine lumen to promote conceptus growth and survival. Chemical inhibition of PG production by both the endometrium and the conceptus prevented elongation in sheep. However, the contributions of conceptus-derived PGs to preimplantation conceptus development remain unclear. In this study, CRISPR-Cas9 genome editing was used to inactivate PTGS2 in ovine embryos to determine the role of PTGS2-derived PGs in conceptus development and elongation. PTGS2 edited conceptuses produced fewer PGs, but secreted similar amounts of IFNT to their Cas9 control counterparts and elongated normally. Expression of PTGS1 was lower in PTGS2 edited conceptuses, but PPARG expression and IFNT secretion were unaffected. Content of PGs in the uterine lumen was similar as was gene expression in the endometrium of ewes who received either Cas9 control or PTGS2 edited conceptuses. These results support the idea that intrinsic PTGS2-derived PGs are not required for preimplantation embryo or conceptus survival and development in sheep.

中文翻译：

---

绵羊植入前绵羊概念的发育不需要前列腺素-过氧化物过氧化物合酶2。

要在反刍动物中成功建立妊娠，必须进行概念发育和伸长，这与干扰素τ（IFNT）和前列腺素（PGs）的产生同时发生。在概念滋养外胚层和子宫内膜中，PGs主要通过前列腺素-过氧化物过氧化物合酶2（PTGS2）途径合成，并修饰子宫内膜的子宫内膜基因表达，从而改变子宫内膜的组织营养成分，从而促进概念生长和存活。子宫内膜和受孕体对PG产生的化学抑制作用阻止了绵羊的伸长。然而，源自概念的PG对植入前概念的发展的贡献仍不清楚。在这项研究中，CRISPR-Cas9基因组编辑用于灭活绵羊胚胎中的PTGS2，以确定PTGS2衍生的PG在概念发育和延长中的作用。PTGS2编辑过的conceptuses产生的PG较少，但分泌的Cas9对照品分泌的IFNτ相似，并且正常伸长。在PTGS2编辑的概念中PTGS1的表达较低，但PPARG表达和IFNτ分泌不受影响。子宫内腔中PG的含量与接受Cas9对照或PTGS2编辑的概念的母羊子宫内膜中的基因表达相似。这些结果支持这样的想法，即内在的PTGS2衍生的PGs不需要植入前的胚胎或绵羊的胎盘存活和发育。子宫内腔中PG的含量与接受Cas9对照或PTGS2编辑的概念的母羊子宫内膜中的基因表达相似。这些结果支持这样的想法，即内在的PTGS2衍生的PGs不需要植入前的胚胎或绵羊的胎盘存活和发育。子宫内腔中PG的含量与接受Cas9对照或PTGS2编辑的概念的母羊子宫内膜中的基因表达相似。这些结果支持这样的想法，即内在的PTGS2衍生的PGs不需要植入前的胚胎或绵羊的胎盘存活和发育。