Introduction EQAS program at New Delhi under IAMM was started in January 2014 across North and North east regions of India with 217 participants, which grew up to 540 by 2018. Materials and Methods In 2014, 4 analytes per year were sent for 3 exercises, i.e. smear culture and serology. 2018 onwards PT analytes were increased from 4 to 12 and comparative performance of techniques analysed. Results Out of the 22 smears sent for gram staining, ZN staining, Kinyoun staining and Albert staining, completely correct results ranged between 29.55% - 79.9%, 94.3% - 99.2%, 35.5% & 93.8%, respectively. Correct results for culture isolate identification & susceptibility testing and serology exercises varied between 70 & 92.4% and 73.1 & 98.59%, respectively. In the year 2018, 470 responses were received for bacterial culture identification & antibiotic susceptibility testing out of which manual and automated systems were used by 54% & 46% and 52.5% & 47.5% participants, respectively. Techniques used in BBV assays for HBsAg, HCV & HIV found all methods like ELISA, ELFA, CLIA and Card Test performing similarly. The major challenges in running the EQA program included requirement of large amount of specimens for PT item preparation, stability in hot and humid conditions and timely delivery of PT challenges in remote parts of the country. Conclusion A large number of the participating laboratories (77%) had an overall score of >80% for all exercises, demonstrating acceptable baseline performance of EQAS registered laboratories. However, continued EQAS participation could further improve the quality of results.

中文翻译：

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印度医学微生物学协会外部质量保证计划中心（新德里）的经验：临床微生物学实验室的挑战和质量评估。


简介 IAMM 下的新德里 EQAS 项目于 2014 年 1 月在印度北部和东北部地区启动，共有 217 名参与者，到 2018 年增加到 540 名。 材料和方法 2014 年，每年发送 4 种分析物进行 3 次练习，即涂片培养和血清学。 2018 年起，PT 分析物从 4 种增加到 12 种，并对分析技术的性能进行比较。结果 送去革兰氏染色、ZN 染色、Kinyoun 染色和 Albert 染色的 22 份涂片中，完全正确率分别为 29.55% - 79.9%、94.3% - 99.2%、35.5% 和 93.8%。培养物分离鉴定、药敏试验和血清学练习的正确结果分别在 70% 和 92.4% 以及 73.1% 和 98.59% 之间变化。 2018 年，共收到 470 份细菌培养鉴定和抗生素敏感性测试回复，其中手动和自动系统分别有 54% 和 46% 以及 52.5% 和 47.5% 的参与者使用。用于 HBsAg、HCV 和 HIV 的 BBV 检测技术发现 ELISA、ELFA、CLIA 和 Card Test 等所有方法的性能相似。运行 EQA 项目的主要挑战包括需要大量样本来准备 PT 项目、在炎热和潮湿条件下的稳定性以及在该国偏远地区及时交付 PT 挑战。结论 大量参与实验室 (77%) 所有练习的总分均超过 80%，表明 EQAS 注册实验室的基线表现可接受。然而，持续参与 EQAS 可以进一步提高结果的质量。