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Resistance profile and molecular characterization of pyrethroid resistance in a Rhipicephalus microplus strain from Colombia.
Medical and Veterinary Entomology ( IF 1.6 ) Pub Date : 2019-11-19 , DOI: 10.1111/mve.12418
D Villar 1 , G M Klafke 2, 3 , A Rodríguez-Durán 4 , F Bossio 1 , R Miller 2 , A A Pérez de León 5 , J A Cortés-Vecino 4 , J J Chaparro-Gutiérrez 1
Affiliation  

Intensive use of chemical acaricides for the control of cattle ticks (Rhipicephalus microplus) has led to the development of multiple acaricide resistance in Colombia. The present study aimed to characterize, using toxicological bioassays and molecular biology techniques, the resistance profile of a tick strain isolated from the Arauca state, Northeast Colombia. Commercial acaricides were used in adult immersion tests to determine its in vitro efficacies. Deltamethrin showed very low activity (4-7.3%), a mixture of cypermethrin and chlorpyrifos had intermediate efficacy (64-75.2%), and ethion presented the highest activity (88.5-100%). A colony (Arauquita strain) was established and larval immersion tests confirmed high resistance level to deltamethrin (241-fold) and susceptibility to ivermectin. A quantitative polymerase chain reaction-high resolution melt technique was used to identify single nucleotide polymorphisms (SNPs) in the para-sodium channel gene. All of the genotyped individuals were mutant, presenting one (n = 7), two (n = 7) or three (n = 9) SNPs previously associated with pyrethroid resistance. Sequencing revealed a novel mutation (F712L), that was found for the first time in R. microplus ticks from South America. This is the first description of mutations associated with pyrethroid resistance in R. microplus from Colombia. The acaricide resistance pattern found in the Arauquita strain is similar to other parts of Colombia.

中文翻译:

哥伦比亚Rhipicephalus microplus菌株中拟除虫菊酯抗性的抗性概况和分子表征。

大量使用化学杀螨剂控制牛cattle(Rhipicephalus microplus)已导致哥伦比亚对多种杀螨剂产生抗药性。本研究旨在使用毒理学生物测定法和分子生物学技术表征从哥伦比亚东北部阿劳卡州分离的壁虱菌株的抗性。商业杀螨剂用于成人浸泡测试,以确定其体外功效。溴氰菊酯显示出极低的活性(4-7.3%),氯氰菊酯和毒死mixture的混合物具有中等效力(64-75.2%),而乙硫胺酸显示出最高的活性(88.5-100%)。建立了一个菌落(Arauquita菌株),幼虫浸没试验证实了对溴氰菊酯的高抗药性(241倍)和对伊维菌素的敏感性。定量聚合酶链反应-高分辨率熔解技术用于鉴定对钠通道基因中的单核苷酸多态性(SNP)。所有基因型个体均为突变体,表现出一个先前与拟除虫菊酯抗性相关的SNP(一个n = 7),两个S(n = 7)或三个Sn(n = 9)。测序揭示了一个新的突变(F712L),该突变是在南美南美微小。中首次发现的。这是对哥伦比亚R. microplus中拟除虫菊酯抗药性相关突变的首次描述。在Arauquita菌株中发现的抗杀螨剂模式与哥伦比亚其他地区相似。以前与拟除虫菊酯抗药性相关的两个(n = 7)或三个(n = 9)SNP。测序揭示了一个新的突变(F712L),该突变是在南美南美微小。中首次发现的。这是对哥伦比亚R. microplus中拟除虫菊酯抗药性相关突变的首次描述。在Arauquita菌株中发现的抗杀螨剂模式与哥伦比亚其他地区相似。以前与拟除虫菊酯抗药性相关的两个(n = 7)或三个(n = 9)SNP。测序揭示了一个新的突变(F712L),该突变是在南美南美微小。中首次发现的。这是对哥伦比亚R. microplus中拟除虫菊酯抗药性相关突变的首次描述。在Arauquita菌株中发现的抗杀螨剂模式与哥伦比亚其他地区相似。
更新日期:2019-11-19
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