A number of complex human diseases including neurological diseases is characterized by dysregulation of long-chain noncoding RNA (lncRNA). The pathogenesis of Alzheimer's disease (AD), a neurodegenerative disorder is believed to involve alterations in lncRNAs. However, the specific lncRNAs modified in AD remain to be determined. The aim of this study was to identify lncRNAs associated with AD using human neuroblastoma cell line (SH-SY5Y) treated with beta-amyloid (Aβ) as a model of this disease. The differential expressions of lncRNA were compared between beta-amyloid (Aβ) SH-SY5Y cells and normal SH-SY5Y cells utilizing Illumina X10 gene sequencing. The differential expression profiles of amyloid (Aβ)-treated SH-SY5Y cells were determined and verified by qRT-PCR method. The expression levels of lncRNA were expressed by calculating the abundance of FPKM (measure gene expression). The differential expression of log2 (multiple change) >1 or log2 (multiple change) < -1 had statistical significance (P< .05). The differential expression profiles of amyloid (Aβ)-treated SH-SY5Y cells showed 40 lncRNA were up-regulated, while 60 lncRNA were down-regulated. GO and KEGG analysis demonstrated that differentially expressed genes were predominantly involved in the mitogen-activated protein kinase (MAPK) signaling pathway, p53 signaling pathway, hepatitis B, cell cycle, post-translational protein modification, and regulation. In conclusion, approximately 100 dysregulated lncRNA transcripts were found in amyloid (Aβ)-treated SH-SY5Y cells and these lncRNAs may play an important role in the occurrence and development of AD through altered signal pathways.

中文翻译：

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人神经母细胞瘤细胞系（SH-SY5Y）：阿尔茨海默氏病细胞模型中差异表达的长链非编码RNA。

许多复杂的人类疾病（包括神经系统疾病）的特征在于长链非编码RNA（lncRNA）的失调。据信阿尔茨海默氏病（AD）是一种神经退行性疾病，其发病机制涉及lncRNA的改变。但是，在AD中修饰的特定lncRNAs尚待确定。这项研究的目的是使用经β-淀粉样蛋白（Aβ）处理的人神经母细胞瘤细胞系（SH-SY5Y）鉴定与AD相关的lncRNAs作为该疾病的模型。利用Illumina X10基因测序技术比较了β-淀粉样蛋白（Aβ）SH-SY5Y细胞和正常SH-SY5Y细胞中lncRNA的差异表达。通过qRT-PCR方法确定并验证了淀粉样蛋白（Aβ）处理的SH-SY5Y细胞的差异表达谱。通过计算FPKM的丰度（测量基因表达）来表达lncRNA的表达水平。log2（多次变化）> 1或log2（多次变化）<-1的差异表达具有统计学意义（P <.05）。淀粉样蛋白（Aβ）处理的SH-SY5Y细胞的差异表达谱显示40个lncRNA被上调，而60个lncRNA被下调。GO和KEGG分析表明差异表达的基因主要参与有丝分裂原激活的蛋白激酶（MAPK）信号通路，p53信号通路，乙型肝炎，细胞周期，翻译后蛋白质修饰和调节。结论，