Tissue engineering vascular grafts (TEVGs) have the potential to replace small-diameter grafts in bypass surgery which is good news for patients with cardiovascular disease. Decellularized arteries can be ideal TEVGs because their natural three-dimensional structures support the migration of host cells and vascular remodeling. There are many methods for decellularization without a standard protocol. In this study, a combination of Triton X-100 and sodium dodecyl sulfate (SDS) were used to prepare decellularized arteries. However, decellularization may damage the biochemical and mechanical properties to some degree. We used the cross-linking agents N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) to improve mechanical properties and immobilize heparin to inhibit thrombogenesis. Histological analysis, scanning electron microscopy, biomechanical properties test, determination of immobilized heparin, active partial thrombin time assay, and subcutaneous embedding experiment were used to evaluate the efficiency of decellularization and the efficacy of heparinized cross-linked vascular scaffold. Results showed 1% Triton X-100 combined with 0.3% SDS can decellularize successfully. EDC and NHS cross-linking can improve the mechanical properties, reduce the inflammatory reaction and slow the degradation time. Heparin immobilized on the scaffolds can inhibit thrombogenesis effectively. This study indicated the heparinized cross-linked vascular scaffolds may be ideal scaffolds for TEVGs.

中文翻译：

---

猪颈动脉的脱细胞，交联和肝素固定化，用于组织工程血管移植。

组织工程血管移植物（TEVGs）在旁路手术中有可能替代小直径移植物，这对于患有心血管疾病的患者来说是个好消息。脱细胞的动脉可能是理想的TEVG，因为它们的天然三维结构支持宿主细胞的迁移和血管重塑。没有标准协议的脱细胞方法有很多。在这项研究中，使用Triton X-100和十二烷基硫酸钠（SDS）的组合来制备脱细胞的动脉。但是，脱细胞作用可能会在一定程度上损害其生化和机械性能。我们使用了交联剂N-（3-二甲基氨基丙基）-N'-乙基碳二亚胺盐酸盐（EDC）和N-羟基琥珀酰亚胺（NHS）来改善机械性能并固定肝素以抑制血栓形成。组织学分析 扫描电镜，生物力学性能测试，固定化肝素的测定，活性部分凝血酶时间测定和皮下包埋实验用于评估脱细胞的效率和肝素化的交联血管支架的功效。结果表明，1％Triton X-100与0.3％SDS结合可以成功脱细胞。EDC和NHS交联可改善机械性能，减少炎症反应并减缓降解时间。固定在支架上的肝素可有效抑制血栓形成。这项研究表明，肝素化的交联血管支架可能是TEVG的理想支架。并通过皮下埋植实验评价脱细胞效率和肝素化交联血管支架的有效性。结果表明，1％Triton X-100与0.3％SDS结合可以成功脱细胞。EDC和NHS交联可改善机械性能，减少炎症反应并减缓降解时间。固定在支架上的肝素可有效抑制血栓形成。这项研究表明，肝素化的交联血管支架可能是TEVG的理想支架。通过皮下埋植实验评价脱细胞效率和肝素化交联血管支架的有效性。结果表明，1％Triton X-100与0.3％SDS结合可以成功脱细胞。EDC和NHS交联可改善机械性能，减少炎症反应并减缓降解时间。固定在支架上的肝素可有效抑制血栓形成。这项研究表明，肝素化的交联血管支架可能是TEVG的理想支架。减少炎症反应并减慢降解时间。固定在支架上的肝素可有效抑制血栓形成。这项研究表明，肝素化的交联血管支架可能是TEVG的理想支架。减少炎症反应并减慢降解时间。固定在支架上的肝素可有效抑制血栓形成。这项研究表明，肝素化的交联血管支架可能是TEVG的理想支架。