In vitro assessments of skin absorption of xenobiotics are essential for toxicological evaluations and bioavailability studies of cosmetic and pharmaceutical ingredients. Since skin metabolism can greatly contribute to xenobiotic absorption, experiments need to be performed with skin explants kept viable in suitable survival media. Existing protocols for non‐viable skin are modified to consider those conditions. The objective was to design a survival medium used as an acceptor fluid in Franz cells for testing cutaneous penetration of hydrophilic or lipophilic molecules. Their metabolism inside skin may be investigated under the same conditions. The determining factors involved in survival mechanisms in vitro are discussed. The consequences of short‐term skin preservation at 4°C were also evaluated.

中文翻译：

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存活受体培养基的配方能够在体外皮肤实验中维持皮肤外植体的活力。

外源性皮肤吸收的体外评估对于化妆品和药物成分的毒理学评估和生物利用度研究至关重要。由于皮肤新陈代谢可以极大地促进外源性物质的吸收，因此需要在合适的生存培养基中保持可行的皮肤外植体进行实验。现有的针对无活力皮肤的方案进行了修改以考虑这些情况。目的是设计一种存活培养基，用作弗朗兹细胞中的受体液，用于测试亲水或亲脂分子的皮肤渗透。可以在相同条件下研究它们在皮肤内的代谢。参与体外存活机制的决定因素被讨论。还评估了 4°C 下短期皮肤保存的后果。