As a member of the S100 protein family, S100A10 has already been purified. However, its purity, or even yield, have often not been reported in the literature. To facilitate future biophysical experiments with S100A10, we aimed to obtain it at a purity of at least 95% in a reasonably large amount. Here, we report optimized conditions for the transformation, overexpression and purification of the protein. We obtained a purity of 97% and performed stability studies by circular dichroism. Our data confirmed that the S100A10 obtained is suitable for experiments to be performed at room temperature up to several days.

中文翻译：

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优化了S100A10的转化，过表达和纯化。

作为S100蛋白家族的成员，S100A10已被纯化。但是，它的纯度，甚至产量，在文献中经常没有报道。为了促进将来使用S100A10进行生物物理实验，我们旨在以合理的数量获得至少95％的纯度。在这里，我们报告了蛋白质转化，过表达和纯化的优化条件。我们获得了97％的纯度，并通过圆二色性进行了稳定性研究。我们的数据证实，所获得的S100A10适合在室温下进行数天的实验。