RNA can be modified in over 100 distinct ways, and these modifications are critical for function. Pseudouridine synthases catalyse pseudouridylation, one of the most prevalent RNA modifications. Pseudouridine synthase 7 modifies a variety of substrates in Saccharomyces cerevisiae including tRNA, rRNA, snRNA, and mRNA, but the substrates for other budding yeast Pus7 homologues are not known. We used CRISPR-mediated genome editing to disrupt Candida albicans PUS7 and find absence leads to defects in rRNA processing and a decrease in cell surface hydrophobicity. Furthermore, C. albicans Pus7 absence causes temperature sensitivity, defects in filamentation, altered sensitivity to antifungal drugs, and decreased virulence in a wax moth model. In addition, we find C. albicans Pus7 modifies tRNA residues, but does not modify a number of other S. cerevisiae Pus7 substrates. Our data suggests C. albicans Pus7 is important for fungal vigour and may play distinct biological roles than those ascribed to S. cerevisiae Pus7.

中文翻译：

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假尿苷合酶7影响白色念珠菌rRNA加工和形态可塑性。

RNA可以100多种不同的方式进行修饰，这些修饰对于功能至关重要。假尿苷合酶催化假尿苷化，这是最普遍的RNA修饰之一。伪尿苷合酶7修饰酿酒酵母中的多种底物，包括tRNA，rRNA，snRNA和mRNA，但是其他萌芽酵母Pus7同源物的底物未知。我们使用CRISPR介导的基因组编辑来破坏白色念珠菌PUS7，发现缺失会导致rRNA加工缺陷和细胞表面疏水性降低。此外，白色念珠菌Pus7的缺失会导致温度敏感性，丝状缺陷，对抗真菌药物的敏感性改变以及蜡蛾模型中毒力降低。此外，我们发现白色念珠菌Pus7修饰了tRNA残基，但没有修饰许多其他S.。啤酒Pus7底物。我们的数据表明，白色念珠菌Pus7对于真菌活力很重要，并且可能比起酿酒酵母Pus7具有更大的生物学作用。