RNA molecules are folded into structures and complexes to perform a wide variety of functions. Determination of RNA structures and their interactions is a fundamental problem in RNA biology. Most RNA molecules in living cells are large and dynamic, posing unique challenges to structure analysis. Here we review progress in RNA structure analysis, focusing on methods that use the "cross-link, proximally ligate, and sequence" principle for high-throughput detection of base-pairing interactions in living cells. Beginning with a comparison of commonly used methods in structure determination and a brief historical account of psoralen cross-linking studies, we highlight the important features of cross-linking methods and new biological insights into RNA structures and interactions from recent studies. Further improvement of these cross-linking methods and application to previously intractable problems will shed new light on the mechanisms of the "modern RNA world."

中文翻译：

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RNA 碱基配对问题和碱基配对解决方案。

RNA 分子折叠成结构和复合物以执行多种功能。RNA 结构及其相互作用的确定是 RNA 生物学的一个基本问题。活细胞中的大多数 RNA 分子都很大且动态，给结构分析带来了独特的挑战。在这里，我们回顾了 RNA 结构分析的进展，重点关注使用“交联、邻近连接和序列”原理高通量检测活细胞中碱基配对相互作用的方法。首先对结构测定中常用的方法进行比较，并简要介绍补骨脂素交联研究的历史，我们重点介绍了交联方法的重要特征以及最近研究中对 RNA 结构和相互作用的新生物学见解。这些交联方法的进一步改进和应用于以前棘手的问题将为“现代RNA世界”的机制提供新的线索。