The fruits of Trapa bispinosa (TB) and seeds of Trigonella foenum-graecum (TF) are used for their nutraceutical properties in various systems of traditional medicine practiced in India. In this study aqueous and methanolic extracts of TB fruits and TF seeds were prepared and their protective effect was studied on hydrogen peroxide (H2O2)-treated neuroblastoma (NB-41) cell line. Cell viability, nitric oxide (NO) levels, mRNA, and protein profiles were analyzed and compared with untreated control. Results show that aqueous extracts of both TB and TF provide significant protection to neuroblastoma cells with TF aqueous extract offering better protection through upregulation of neuronal nitric oxide synthase (nNOS) against hydrogen peroxide generated stress. This is the first report demonstrating protection of neuronal cell lines by use of TB and TF extracts through modulation of nNOS activity.

中文翻译：

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通过神经元一氧化氮合酶的捕蝇草和三角叶草对神经母细胞瘤细胞的保护和调节作用。

在印度实行的各种传统医学系统中，使用Trapa bispinosa（TB）的果实和Trigonella foenum-graecum（TF）的种子作为营养品。本研究制备了TB果实和TF种子的水和甲醇提取物，并研究了它们对过氧化氢（H2O2）处理的神经母细胞瘤（NB-41）细胞系的保护作用。分析细胞活力，一氧化氮（NO）水平，mRNA和蛋白质谱并将其与未处理的对照进行比较。结果表明，TB和TF的水提取物对神经母细胞瘤细胞提供了显着的保护，而TF水提取物通过上调神经元一氧化氮合酶（nNOS）抵抗过氧化氢产生的压力而提供了更好的保护。