Introduction: The LTA and TNFA genes encode key proinflammatory cytokines with diverse activities in the immune responses. Particular single nucleotide polymorphisms (SNPs) in the LTA rs909253 (+252 A > G) and TNFA rs1800629 (-308 G > A) genes have been associated with susceptibility to many complex diseases. The aim of this study was to assess the frequency for these two key polymorphisms in the Moroccan population. Materials and Methods: A total of 338 unrelated healthy Moroccan subjects were genotyped for the two genes using a restriction fragment length polymorphism-polymerase chain reaction method. Results: The LTA (+252 A > G) and TNFA (-308 G > A) were the most common alleles with 67.9% and 74.8% frequencies, respectively. In addition to the linkage disequilibrium between the two SNPs, significant differences in allele frequencies were observed in Moroccan population compared with Mediterraneans, Europeans, Africans, South Americans, and Asians (p < 0.05). Finally, genetic proximities between Moroccan, European, and West African populations were found by means of the principal component analysis. Conclusion: The LTA +252 A>G and TNFA -308 G > A polymorphisms among Moroccan population follow the patterns commonly encountered in other Mediterranean, European, and African populations. The result of this study could contribute in developing a genetic database on the healthy Moroccan population.

中文翻译：

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LTA +252 A> G和TNFA -308 G> A在摩洛哥人群中的多态性的遗传分布。

简介：LTA和TNFA基因编码关键的促炎细胞因子，在免疫反应中具有多种活性。LTA rs909253（+252 A> G）和TNFA rs1800629（-308 G> A）基因中的特定单核苷酸多态性（SNP）与许多复杂疾病的易感性相关。这项研究的目的是评估摩洛哥人口中这两个关键多态性的频率。材料和方法：使用限制性片段长度多态性-聚合酶链反应法对总共338名摩洛哥健康无关个体的两个基因进行基因分型。结果：LTA（+252 A> G）和TNFA（-308 G> A）是最常见的等位基因，频率分别为67.9％和74.8％。除了两个SNP之间的连锁不平衡，与地中海人，欧洲人，非洲人，南美人和亚洲人相比，摩洛哥人的等位基因频率存在显着差异（p <0.05）。最后，通过主成分分析发现了摩洛哥，欧洲和西非人口之间的遗传邻近性。结论：摩洛哥人群中的LTA +252 A> G和TNFA -308 G> A多态性遵循其他地中海，欧洲和非洲人群中常见的模式。这项研究的结果可能有助于开发健康的摩洛哥人口的遗传数据库。通过主成分分析发现了西非人口。结论：摩洛哥人群中的LTA +252 A> G和TNFA -308 G> A多态性遵循其他地中海，欧洲和非洲人群中常见的模式。这项研究的结果可能有助于开发健康的摩洛哥人口的遗传数据库。通过主成分分析发现了西非人口。结论：摩洛哥人群中的LTA +252 A> G和TNFA -308 G> A多态性遵循其他地中海，欧洲和非洲人群中常见的模式。这项研究的结果可能有助于开发健康的摩洛哥人口的遗传数据库。