Mechanical stimuli influence various physiological processes in osteoblasts. We previously showed that mechano-growth factor (MGF), a splicing variant of insulin-like growth factor 1, is highly expressed in osteoblasts in response to mechanical stimuli. This study aims to explore the systemic functions of MGF in osteoblasts, and the mechanisms by which mechanical stress regulates the alternative splicing of Igf1 to generate MGF. We found that MGF promoted the proliferation and migration of osteoblasts, while it inhibited their differentiation via Erk1/2 pathway. Furthermore, cyclic stretching upregulated the expression of ASF/SF2, which in turn regulated the expression of MGF. Our findings indicate that mechanical stimuli influence the physiological responses of osteoblasts by increasing the expression of MGF, which is regulated by splicing factors.

中文翻译：

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在机械刺激下，剪接因子调节的机械生长因子的产生调节成骨细胞的生理过程。

机械刺激影响成骨细胞的各种生理过程。我们以前显示机械生长因子（MGF），胰岛素样生长因子1的剪接变体在成骨细胞中响应机械刺激而高表达。这项研究旨在探讨成骨细胞中MGF的系统功能，以及机械应力调节Igf1选择性剪接生成MGF的机制。我们发现MGF促进成骨细胞的增殖和迁移，同时通过Erk1 / 2途径抑制其分化。此外，循环拉伸上调了ASF / SF2的表达，进而调节了MGF的表达。我们的发现表明，机械刺激通过增加MGF的表达影响成骨细胞的生理反应，