Dynamical features of cell signaling are the essence of living organisms. To understand animal development, it is fundamental to investigate signaling dynamics in vivo. Robust reporters are required to visualize spatial and temporal dynamics of enzyme activities and protein-protein interactions involved in signaling pathways. In this review, we summarize recent development in the design of new classes of fluorescent reporters for imaging dynamic activities of proteases, kinases, and protein-protein interactions. These reporters operate on new physical and/or chemical principles; achieve large dynamic range, high brightness, and fast kinetics; and reveal spatiotemporal dynamics of signaling that is correlated with developmental events such as embryonic morphogenesis in live animals including Drosophila and zebrafish. Therefore, many of these reporters are great tools for biological discovery and mechanistic understanding of animal development and disease progression.

中文翻译：

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使用新型荧光报告分子在体内对动态细胞信号进行成像。

细胞信号传导的动态特征是生命有机体的本质。要了解动物的发育，研究体内信号动力学至关重要。需要强大的报告者来可视化信号通路中涉及的酶活性和蛋白相互作用的时空动态。在这篇综述中，我们总结了新型荧光报告基因设计的最新进展，这些新型荧光报告基因用于成像蛋白酶，激酶和蛋白质-蛋白质相互作用的动态活性。这些记者采用新的物理和/或化学原理；实现大动态范围，高亮度和快速动力学；并揭示与发育事件（例如果蝇和斑马鱼）中的胚胎形态发生等发育事件相关的信号时空动态。因此，