The potato/tomato psyllid Bactericera cockerelli causes serious damage to several solanaceous crops by direct feeding and vectoring Candidatus Liberibacter solanacearum, a bacterial pathogen. Electron beam (eBeam) irradiation is an environmentally friendly, chemical‐free alternative method that is increasing in use for disinfestation of insect pests. We hypothesize that this irradiation technology will have detrimental effects on potato psyllid and thus impede its disease vectoring. To this end, we explored the effects of eBeam treatment ranging from 50 to 500 Gy on survival, development and reproduction of this pest. Impact on psyllids was apparently dose‐dependent. When irradiated at 350 Gy, eggs could not hatch, 1st instar nymphs failed to emerge, and although a small portion of irradiated 5th instar nymphs survived, the emerged adults were mostly deformed. Abnormality in eclosed adults suggests harmful effects of eBeam on metamorphosis. Reproduction was seriously impaired when female psyllids were exposed to eBeam at the 5th instar nymphal or young adult stage, presumably due to inability to form oocytes. In addition, reciprocal crosses between irradiated and untreated psyllids indicated that female psyllids were more radiosensitive than males to eBeam. Taken together, these findings indicate that eBeam negatively impacted potato psyllid development and reproduction, which would inevitably compromise its disease transmission capacity. A dose of 350 Gy can be considered as a reference dose for effective control of potato psyllids.

中文翻译：

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电子束辐照对番茄/马铃薯木虱、可卡氏杆菌发育和繁殖的有害影响

马铃薯/番茄木虱 Bactericera cockerelli 通过直接饲喂和传播 Candidatus Liberibacter solanacearum（一种细菌病原体）对几种茄科作物造成严重损害。电子束 (eBeam) 辐照是一种环保、无化学物质的替代方法，越来越多地用于灭虫。我们假设这种辐照技术将对马铃薯木虱产生不利影响，从而阻碍其病媒传播。为此，我们研究了 50 到 500 Gy 的电子束处理对这种害虫的生存、发育和繁殖的影响。对木虱的影响显然是剂量依赖性的。350 Gy辐照时，卵不能孵化，1龄若虫未能出现，虽然一小部分辐照5龄若虫存活下来，出现的成年人大多是畸形的。eclosed 成虫的异常表明 eBeam 对变形的有害影响。当雌性木虱在 5 龄若虫或年轻成人阶段暴露于 eBeam 时，繁殖受到严重损害，这可能是由于无法形成卵母细胞。此外，辐照和未处理木虱之间的相互交叉表明，雌性木虱对 eBeam 的辐射敏感性高于雄性。总之，这些发现表明 eBeam 对马铃薯木虱的发育和繁殖产生了负面影响，这将不可避免地损害其疾病传播能力。350 Gy 的剂量可作为有效控制马铃薯木虱的参考剂量。当雌性木虱在 5 龄若虫或年轻成人阶段暴露于 eBeam 时，繁殖受到严重损害，这可能是由于无法形成卵母细胞。此外，辐照和未处理木虱之间的相互交叉表明，雌性木虱对 eBeam 的辐射敏感性高于雄性。总之，这些发现表明 eBeam 对马铃薯木虱的发育和繁殖产生了负面影响，这将不可避免地损害其疾病传播能力。350 Gy 的剂量可作为有效控制马铃薯木虱的参考剂量。当雌性木虱在 5 龄若虫或年轻成人阶段暴露于 eBeam 时，繁殖受到严重损害，这可能是由于无法形成卵母细胞。此外，辐照和未处理木虱之间的相互交叉表明，雌性木虱对 eBeam 的辐射敏感性高于雄性。总之，这些发现表明 eBeam 对马铃薯木虱的发育和繁殖产生了负面影响，这将不可避免地损害其疾病传播能力。350 Gy 的剂量可作为有效控制马铃薯木虱的参考剂量。辐照和未处理木虱之间的相互交叉表明，雌性木虱比雄性对电子束更敏感。总之，这些发现表明 eBeam 对马铃薯木虱的发育和繁殖产生了负面影响，这将不可避免地损害其疾病传播能力。350 Gy 的剂量可作为有效控制马铃薯木虱的参考剂量。辐照和未处理木虱之间的相互交叉表明，雌性木虱比雄性对电子束更敏感。总之，这些发现表明 eBeam 对马铃薯木虱的发育和繁殖产生了负面影响，这将不可避免地损害其疾病传播能力。350 Gy 的剂量可作为有效控制马铃薯木虱的参考剂量。