The aquaculture growth can be followed by the occurrence of more and new pathogenic agents, since the production leads to higher fish densities in confined areas more appropriate to the appearance and propagation of pathologies. Copper sulfate has been widely used in preventing and controlling fish parasites. The objective of this study is to investigate the effects of copper treatments in the fish tissues (bioaccumulation and histological changes in different organs), mortality and evaluate what happens during the recovery period. White sea bream (Diplodus sargus) were exposed to copper sulfate (0.25 and 0.5 mg L-1) during 60 days followed with a 75-day recovery period. The results showed that the concentration of copper in fish liver was significantly higher in the 0.5 mg L-1 treatment than in the 0.25 mg L-1 treatment. Conversely, copper load in the muscle did not differ significantly between treatments and control. Copper levels in muscle, and especially in liver, increased during copper exposure (up to 60 days). In summary, at higher concentrations copper sulfate treatment (0.5 mg L-1) might be toxic to fish, which showed histological alterations and copper accumulation in their tissues, mainly in the liver. Nevertheless, individuals returned to their original state after a 75-day recovery period and the tested copper concentrations does not represents risk for food safety.

中文翻译：

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饲养的梁龙的铜暴露和恢复期的影响。

水产养殖的增长可以伴随着更多新病原体的发生，因为这种生产导致密闭地区的鱼类密度更高，更适合病态的出现和传播。硫酸铜已被广泛用于预防和控制鱼类寄生虫。这项研究的目的是调查铜处理对鱼组织的影响（不同器官的生物积累和组织学变化），死亡率，并评估恢复期发生的情况。在60天内，将白鲷（Diplodus sargus）暴露于硫酸铜（0.25和0.5 mg L-1）中，然后恢复75天。结果表明，鱼肝中的铜浓度在0.5 mg L-1处理中明显高于0.25 mg L-1处理。反过来，在治疗和对照之间，肌肉中的铜负荷没有显着差异。在暴露于铜的过程中（长达60天），肌肉（尤其是肝脏）中的铜含量增加。总之，在较高浓度下，硫酸铜（0.5 mg L-1）处理可能对鱼类有毒，这表明其组织（主要是肝脏）的组织学改变和铜积累。然而，经过75天的恢复期，个体恢复到原始状态，测试的铜浓度不代表食品安全风险。表现出组织学改变和组织（主要在肝脏）中的铜积累。然而，经过75天的恢复期，个体恢复到原始状态，测试的铜浓度不代表食品安全风险。表现出组织学改变和组织（主要在肝脏）中的铜积累。然而，经过75天的恢复期，个体恢复到原始状态，测试的铜浓度不代表食品安全风险。