Karyotypes of 3 male Talpa specimens from northern Spain were analyzed. The mesostyles of upper molars and cytochrome b sequence analysis identified these specimens as belonging to Talpa aquitania, a new Talpa species recently described from northern Spain and southern France. We describe here for the first time the karyotype of Talpa aquitania. Its diploid number is 2n = 34 and NFa = 64, and all chromosomes including the sex chromosomes are biarmed, either metacentric or submetacentric. G-banding demonstrated that the karyotypes of T. aquitania and T. occidentalis (the most closely related species) are almost identical. However, the karyotype of T. aquitania differs from the karyotypes of both T. europaea and T. occidentalis in that it has a medium-sized biarmed Y chromosome rather than a dot-like chromosome and that chromosome 16 is submetacentric in T. aquitania but has a small p-arm in both T. europaea and T. occidentalis. Pericentromeric C-bands were scarce and only clearly visible in a few chromosomal pairs. In addition, C-banding demonstrated that half of the 14p, the 16p, and the Y chromosome are all heterochromatic. rDNA genes were located at the secondary constriction in autosomal pair 3, a common feature in the karyotypes of all Talpa species. Hybridization signals for telomeric repeats were found on the telomeres and the pericentric regions of some chromosomes and co-localized in the secondary constriction of pair 3 with the rDNA genes. In conclusion, the karyotype of T. aquitania from northern Spain is very similar to the karyotype of other species belonging to the genus Talpa.

中文翻译：

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来自西班牙北部的新 Talpa 物种 Talpa aquitania (Talpidae; Insectivora) 的核型分析

分析了来自西班牙北部的 3 个雄性 Talpa 标本的核型。上臼齿的中柱和细胞色素 b 序列分析确定这些标本属于 Talpa aquitania，这是最近在西班牙北部和法国南部描述的一种新的 Talpa 物种。我们在这里首次描述了 Talpa aquitania 的核型。它的二倍体数为 2n = 34 和 NFa = 64，包括性染色体在内的所有染色体都是双臂的，无论是中着丝粒还是亚中着丝粒。G 显带证明 T. aquitania 和 T. occidentalis（最密切相关的物种）的核型几乎相同。然而，T. aquitania 的核型不同于 T. europaea 和 T. occidentalis，因为它具有中等大小的双臂 Y 染色体而不是点状染色体，并且 16 号染色体在 T. aquitania 中是亚中心的，但在 T. europaea 和 T. occidentalis 中都有一个小的 p 臂。着丝粒周围 C 带很少，仅在少数染色体对中清晰可见。此外，C 显带证明 14p、16p 和 Y 染色体的一半都是异色的。rDNA 基因位于常染色体对 3 的次级缢缩处，这是所有 Talpa 物种核型的共同特征。在端粒和一些染色体的着丝粒周围区域发现了端粒重复的杂交信号，并与 rDNA 基因共定位在第 3 对的二级收缩区。总之，T的核型。