Endocrine-disrupting chemicals have been known to alter important animal behaviors by modulating serotonin (5-hydroxytryptamine, 5-HT) and dopamine. F. heteroclitus (mummichog) brain serotonin and dopamine levels were quantified by enzyme-linked immunosorbent assay (ELISA) following a 28-day exposure regimen involving daily doses of either 0.1 mg l-1 benzyl butyl phthalate (BBP) dissolved in acetone or acetone alone (0.1 mg l-1). No differences in mean brain mass or total protein homogenate were induced by exposure to the acetone vehicle or BBP in acetone. The acetone vehicle had no effect on dopamine, serotonin, or tyrosine hydroxylase levels, but acetone did decrease tryptophan hydroxylase levels (p = 0.011). Exposure to BBP in acetone decreased dopamine (p = 0.024), increased serotonin (p < 0.001), reduced tryptophan hydroxylase as compared to the acetone vehicle alone (p < 0.001), and had no significant effect on tyrosine hydroxylase levels. This study is the first to report modulation of F. heteroclitus brain serotonin and its enzyme tryptophan hydroxylase following sub-lethal exposure to BBP in an acetone vehicle. In addition, modulation of brain dopamine in F. heteroclitus, sans simultaneous modulation of tyrosine hydroxylase, was also observed. These findings support the use of F. heteroclitus for assessing sub-lethal BBP exposure.

中文翻译：

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异体胃底（mummichog）中邻苯二甲酸苄基丁酯对大脑5-羟色胺的调节。

已知破坏内分泌的化学物质可通过调节血清素（5-羟色胺，5-HT）和多巴胺来改变重要的动物行为。在28天的暴露方案后，通过酶联免疫吸附测定（ELISA）定量测定异形沙门氏菌（mummichog）脑中5-羟色胺和多巴胺的水平，该方案涉及每日剂量的溶于丙酮或丙酮中的0.1 mg -1邻苯二甲酸苄基丁酯（BBP）的日剂量单独使用（0.1 mg l-1）。通过暴露于丙酮中的丙酮媒介物或BBP，平均脑质量或总蛋白匀浆没有差异。丙酮媒介物对多巴胺，5-羟色胺或酪氨酸羟化酶水平没有影响，但丙酮确实会降低色氨酸羟化酶水平（p = 0.011）。丙酮中BBP的暴露会降低多巴胺（p = 0.024），血清素增加（p <0.001），与单独的丙酮媒介物相比，色氨酸羟化酶降低了（p <0.001），并且对酪氨酸羟化酶水平没有显着影响。这项研究是第一个报告亚致死性暴露于丙酮载体中的BBP后，对杂种镰刀菌脑5-羟色胺及其色氨酸羟化酶的调节的研究。另外，还观察到了在异形镰刀菌中脑多巴胺的调节，没有酪氨酸羟化酶的同时调节。这些发现支持异形镰刀菌用于评估亚致死性BBP暴露。还观察到了在异形镰刀菌中脑多巴胺的调节，没有酪氨酸羟化酶的同时调节。这些发现支持异形镰刀菌用于评估亚致死性BBP暴露。还观察到了在异形镰刀菌中脑多巴胺的调节，没有酪氨酸羟化酶的同时调节。这些发现支持异形镰刀菌用于评估亚致死性BBP暴露。