The use of bioengineered skin has facilitated fundamental and applied research because it enables the investigation of complex interactions between various cell types as well as the extracellular matrix. The predominantly manual fabrication of these living tissues means, however, that their quality, standardization, and production volume are extremely dependent on the technician's experience. Simple laboratory automation could facilitate the use of living tissues by a greater number of research groups. We developed and present here an injection molding technique for the fabrication of bilayered skin equivalents. The tissue was formed automatically by two separate injections into a customized mold to produce the dermal and epidermal skin layers. We demonstrated the biocompatibility of this fabrication process and confirmed the resulting bilayered morphology of the bioengineered skin using histology and immunohistochemistry. Our findings highlight the possibility of fabricating multilayered living tissue by injection molding, suggesting that further investigation into this automation method could result in the rapid and low-cost fabrication of standardized bioengineered skin.

中文翻译：

---

通过注塑成型制造生物工程皮肤：自动化的可行性研究。

生物工程皮肤的使用促进了基础研究和应用研究，因为它可以研究各种细胞类型以及细胞外基质之间的复杂相互作用。然而，这些活组织的主要手工制造意味着它们的质量，标准化和生产量在很大程度上取决于技术人员的经验。简单的实验室自动化可以促进大量研究小组使用活组织。我们在这里开发并介绍了一种用于制造双层皮肤等效物的注射成型技术。通过两次单独注射到定制的模具中以自动形成组织，以生产真皮和表皮皮肤层。我们证明了这种制造过程的生物相容性，并使用组织学和免疫组织化学方法证实了生物工程皮肤的双层形态。我们的发现凸显了通过注射成型制造多层活体组织的可能性，这表明对该自动化方法的进一步研究可能会导致快速，低成本地制造标准化的生物工程皮肤。