Phospholipase A and acyltransferase 4 (PLAAT4) is a member of the HREV107 tumor suppressor gene family. The expression of PLAAT4 has been shown to induce cell death; however, the underlying mechanism remains unknown. Here, we found that RPLP0, a ribosomal protein, can interact with PLAAT4, as determined by yeast two-hybrid screening, coimmunoprecipitation, and colocalization. The level of RPLP0 was suppressed in HtTA cervical cancer cells expressing PLAAT4. In PLAAT4-expressing or RPLP0-silenced cells, decreased cell viability and cell proliferation combined with increased cell death were observed. Furthermore, the levels of cell cycle-associated proteins and anti-apoptotic proteins decreased in PLAAT4-expressing or RPLP0-silenced cells. Similar patterns of cell viability and expression levels of cell-cycle-associated proteins and apoptosis-related proteins were observed in PLAAT4-expressing and RPLP0-knockdown cells, indicating that RPLP0 deficiency might be involved in PLAAT4-mediated growth inhibition and cellular apoptosis.

中文翻译：

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核糖体蛋白RPLP0介导PLAAT4诱导的细胞周期阻滞和细胞凋亡。

磷脂酶A和酰基转移酶4（PLAAT4）是HREV107肿瘤抑制基因家族的成员。已经显示PLAAT4的表达可诱导细胞死亡。但是，其潜在机制仍然未知。在这里，我们发现RPLP0（一种核糖体蛋白）可以与PLAAT4相互作用，这是通过酵母双杂交筛选，共免疫沉淀和共定位确定的。在表达PLAAT4的HtTA宫颈癌细胞中RPLP0的水平受到抑制。在表达PLAAT4或RPLP0沉默的细胞中，观察到细胞活力和细胞增殖下降，同时细胞死亡增加。此外，在表达PLAAT4或RPLP0沉默的细胞中，细胞周期相关蛋白和抗凋亡蛋白的水平降低。