Abstract

In recent years, herbal tea consumption becomes popular because of the potential health benefits and attractive flavors. However, there is also a growing concern that herbal supplements contribute to the drug-drug/drug-herb interactions and hepatotoxicity. In this study, FL83B mouse hepatocytes were used as an in vitro mode of hepatotoxicity induced by free fatty acids, including palmitic acid (PA) and oleic acid (OA), ethanol, and acetaminophen. Herbal tea extracts were obtained from eight common herbal plants, including Verbena officinalis L., Hyssopus officinalis L., Salvia officinalis L., Urtica dioica L., Hemerocallis fulva (L.) L., Citrus maxima (Burm.) Merr., Citrus limon (L.) Osbeck, and Ficus formosana Maxim. MTT assay was used to evaluate the impact of these herbal tea extracts on hepatoxocitity. We found that these herbal tea extracts per se did not exhibit hepatotoxicity, and had no effect on OA-induced hepatotoxicity. However, extracts from Verbena officinalis L., Hyssopus officinalis L., Salvia officinalis L., and Hemerocallis fulva (L.) L. exhibited protective effect against PA-induced hepatotoxicity. In addition, herbal tea extracts from Verbena officinalis L., Hyssopus officinalis L., Salvia officinalis L., Urtica dioica L., Hemerocallis fulva (L.) L., and Ficus formosana Maxim. exhibited protective effect against acetaminophen-induced hepatotoxicity. Interestingly, all these herbal tea extracts enhanced ethanol-induced hepatotoxicity. Our results suggest that herbal tea extracts have differential effects on different modes of hepatotoxicity.

摘要

近年来，由于潜在的健康益处和诱人的口味，凉茶消费变得流行。然而，人们也越来越担心草药补充剂会导致药物-药物/药物-草药相互作用和肝毒性。在这项研究中，FL83B 小鼠肝细胞被用作游离脂肪酸（包括棕榈酸 (PA) 和油酸 (OA)、乙醇和对乙酰氨基酚）诱导的肝毒性的体外模式。凉茶提取物取自八种常见的草本植物，包括马鞭草、海索草、丹参、荨麻、萱草(L.) L.、Citrus maxima(Burm.) Merr.、Citrus limon (L.) Osbeck 和Ficus formosana Maxim。MTT 法用于评估这些凉茶提取物对肝毒性的影响。我们发现这些凉茶提取物本身没有表现出肝毒性，并且对 OA 诱导的肝毒性没有影响。然而，马鞭草、Hyssopus officinalis L.、Salvia officinalis L. 和Hemerocallis fulva (L.) L.的提取物对 PA 诱导的肝毒性具有保护作用。此外，从马鞭草、海索草、鼠尾草中提取的凉茶提取物，Urtica dioica L.、Hemerocallis fulva (L.) L. 和Ficus formosana Maxim。对乙酰氨基酚引起的肝毒性具有保护作用。有趣的是，所有这些凉茶提取物都增强了乙醇诱导的肝毒性。我们的研究结果表明，凉茶提取物对不同模式的肝毒性有不同的影响。