ABSTRACT

Pseudomonas aeruginosa is a major public health concern all around the world. In the frame of this work, a set of diverse environmental P. aeruginosa isolates with various antibiotic resistance profiles were examined in a Galleria mellonella virulence model. Motility, serotypes, virulence factors and biofilm-forming ability were also examined. Molecular types were determined by pulsed-field gel electrophoresis (PFGE). Based on our results, the majority of environmental isolates were virulent in the G. mellonella test and twitching showed a positive correlation with mortality. Resistance against several antibiotic agents such as Imipenem correlated with a lower virulence in the applied G. mellonella model. PFGE revealed that five examined environmental isolates were closely related to clinically detected pulsed-field types. Our study demonstrated that industrial wastewater effluents, composts, and hydrocarbon-contaminated sites should be considered as hot spots of high-risk clones of P. aeruginosa.

摘要

铜绿假单胞菌是全世界主要的公共卫生问题。在这项工作的框架内，在大蜡螟毒力模型中检查了一组具有各种抗生素抗性特征的不同环境铜绿假单胞菌分离株。还检查了运动性、血清型、毒力因子和生物膜形成能力。通过脉冲场凝胶电泳 (PFGE) 确定分子类型。根据我们的结果，大多数环境分离株在G. mellonella测试中是有毒的，抽搐与死亡率呈正相关。对几种抗生素药物（如亚胺培南）的耐药性与应用的G. mellonella 中较低的毒力相关模型。PFGE 显示，五个经过检查的环境分离株与临床检测到的脉冲场类型密切相关。我们的研究表明，工业废水、堆肥和碳氢化合物污染场地应被视为铜绿假单胞菌高风险克隆的热点。