Arsenic (As) accumulation in rice is a principal route of As exposure for rice based population. We have tested physiochemical and molecular parameters together to identify low As accumulating rice cultivars with normal growth and vigor. The present study examined potential toxicity caused by arsenate (AsV) among four rice cultivars tested that varied with respect to accumulation of total arsenic, arsenite (AsIII) and their differential translocation rate which had deleterious impact on growth and metabolism. Intracellular homeostasis of rice cultivars viz., TN-1, IR-64, IR-20 and Tulaipanji was hampered by 21 days long As(V) treatment due to generation of reactive oxygen species (ROS) and inadequate activity of catalase (CAT; EC 1.11.1.6). Upregulation of oxidative stress markers viz., H2O2, proline and MDA along with alteration in enzymatic antioxidants profile were conspicuously pronounced in cv. Tulaipanji while cv. TN-1 was least affected under As(V) challenged environment. In addition to that genomic template stability and band sharing indices were qualitatively measured by DNA profiling of all tested cultivars treated with 25 μM, 50 μM, and 75 μM As(V). In rice cv. Tulaipanji genetic polymorphism was significantly detected with the application of random amplified polymorphic DNA (RAPD) tool and characterized as susceptible cultivar of As compared to cvs. TN-1, IR-64 and IR-20 that is in correlation with data obtained from cluster analysis. Hence, identified As tolerant cultivars viz., TN-1, IR64 and IR-20 especially TN-1 could be used in As contaminated agricultural field after appropriate field trial. This study could help to gather information regarding cultivar-specific tolerance strategy to avoid pollutant induced toxicity.

中文翻译：

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基于砷积累，易位及其对in稻品种理化变化和基因组不稳定性的影响，评价砷诱导的毒性。

稻米中砷的积累是稻米人群砷暴露的主要途径。我们已经对生理化学和分子参数进行了测试，以确定具有正常生长和活力的低砷积累型水稻品种。本研究检查了四个受试水稻品种中砷（AsV）引起的潜在毒性，这些砷在总砷，砷（AsIII）的累积及其差异转运速率方面均发生变化，这对生长和代谢产生有害影响。由于活性氧（ROS）的产生和过氧化氢酶（CAT; CAT）的活性不足，TN 21，TN-1，IR-64，IR-20和图莱潘基水稻品种的细胞内稳态受到21天的As（V）处理的阻碍。 EC 1.11.1.6）。氧化应激标志物，H2O2，在简历中，脯氨酸和丙二醛含量显着升高，同时酶抗氧化剂的分布也发生了明显变化。图莱潘基同时担任简历。TN-1在As（V）挑战环境下受影响最小。除此以外，基因组模板的稳定性和谱带共享指数是通过对用25μM，50μM和75μMAs（V）处理的所有测试品种的DNA谱进行定性测量的。在大米简历中。通过使用随机扩增多态性DNA（RAPD）工具可显着检测到Tulaipanji遗传多态性，其特征是与cvs相比，As的易感品种。TN-1，IR-64和IR-20与从聚类分析获得的数据相关。因此，经过适当的田间试验后，鉴定出的耐砷品种，即TN-1，IR64和IR-20，尤其是TN-1可以用于As污染的农业领域。