SummaryProchilodus brevis is a rheophilic species with a threatened natural population that promotes studies aimed at optimizing reproduction in captivity. The correct quantity of inseminating dose and activating solution volume significantly improves fertilization rates, thereby increasing productivity in captivity. The objective of this study was to determine the proportion of sperm per oocyte and the ideal volume of activating solution to be used in the assisted fertilization of P. brevis. Gametes were collected and fertilization performed in two steps. In step 1, the ideal proportion of spermatozoa was determined based on the fertilization rate:oocyte by testing six doses of semen: D1 = 30 × 103, D2 = 150 × 103, D3 = 300 × 103, D4 = 3 × 106, D5 = 5 × 106, and D6 = 10 × 106. In step 2, the fertilization and hatching rates were evaluated using different volumes of activating solution (V1 – 25 ml, V2 – 50 ml, V3 – 75 ml,V4 – 100 ml, V5 – 125 ml, and V6 – 150 ml). A linear regression equation was estimated from steps 1 and 2. The Student–Newman–Keuls test was used to compare the means. In step 1, the percentage of fertilization increased linearly, reaching a plateau of 51.69%. In step 2, the best fertilization rates were obtained with an estimated ideal volume of 75.64 ml per 2 ml of oocytes. Therefore, the proportion of 928,410.29 sperm:oocyte, associated with the volume of 75.64 ml of water per 2 ml of oocytes, provided the maximum reproductive performance for P. brevis.

中文翻译：

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短吻鲈受精最佳活化液体积和最佳精子比例的确定

概括短吻鲶是一种自然种群受到威胁的嗜流性物种，促进了旨在优化圈养繁殖的研究。正确的授精剂量和活化溶液体积显着提高了受精率，从而提高了圈养的生产力。本研究的目的是确定每个卵母细胞中精子的比例以及用于辅助受精的活化溶液的理想体积。P. brevis. 收集配子并分两步进行受精。第一步，根据受精率确定理想的精子比例：通过测试六剂精液的卵母细胞：D1 = 30 × 103, D2 = 150 × 103, D3 = 300 × 103, D4 = 3 × 106, D5 = 5 × 106, D6 = 10 × 106. 在步骤 2 中，使用不同体积的活化溶液（V1 – 25 ml、V2 – 50 ml、V3 – 75 ml、V4 – 100 ml、V5 – 125 ml 和 V6 – 150 ml）评估受精率和孵化率。从步骤 1 和 2 估计线性回归方程。Student-Newman-Keuls 检验用于比较平均值。在第 1 步中，受精百分比线性增加，达到 51.69% 的平台期。在第 2 步中，获得最佳受精率，估计理想体积为每 2 毫升卵母细胞 75.64 毫升。因此，928,410.29 个精子：卵母细胞的比例，与每 2 毫升卵母细胞 75.64 毫升水的体积相关，提供了最大的生殖性能P. brevis.