The long noncoding RNA CARLo-5 is dysregulated in multiple types of human cancers. High CARLo-5 is a promising predictive factor for various cancers, including endometrial carcinoma (EC). Our previous study showed that the expression level of CARLo-5 was associated with advanced FIGO stage (The International Federation of Gynecology and Obstetrics), lymph node metastasis, and the poor survival of patients with EC. In the present study, we demonstrated that the downregulation of CARLo-5 could affect the proliferation, cell cycle, migration, and invasion of EC cell lines HEC-1B and KLE cells. The oncogenic activity of CARLo-5 was also confirmed with in vivo data. Mechanistically, CARLo-5 could affect the expression of CDK/CDKN1A and MMP2/9, which have been reported to be regulated by CARLo-5 and associated with cell cycle and motility. In conclusion, this study is the first to discover the biological function and mechanism of CARLo-5 in regulating the biological characteristics of EC cells. Targeting CARLo-5 and its pathway might provide new biomarkers or potential therapies target for patients with EC. Environ. Mol. Mutagen. 61:256-265, 2020. © 2019 Wiley Periodicals, Inc.

中文翻译：

---

CARLo-5是子宫内膜癌的致癌基因。

长的非编码RNA CARLo-5在多种类型的人类癌症中失调。高CARLo-5是各种癌症（包括子宫内膜癌（EC））的有前途的预测因素。我们以前的研究表明，CARLo-5的表达水平与FIGO晚期（国际妇产科联合会），淋巴结转移和EC患者生存不良有关。在本研究中，我们证明了CARLo-5的下调可能影响EC细胞系HEC-1B和KLE细胞的增殖，细胞周期，迁移和侵袭。体内数据也证实了CARlo-5的致癌活性。从机理上讲，CARLo-5可能影响CDK / CDKN1A和MMP2 / 9的表达，据报道这些CDK / CDKN1A和MMP2 / 9受CARLo-5调节，并与细胞周期和运动性有关。结论，这项研究是第一个发现CARLo-5在调节EC细胞生物学特性中的生物学功能和机制的研究。靶向CARLo-5及其途径可能为EC患者提供新的生物标志物或潜在的治疗靶标。环境。大声笑 诱变剂。61：256-265，2020.©2019 Wiley Periodicals，Inc.