We used single-cell RNA sequencing (seq) on several human induced pluripotent stem (iPS) cell-derived neural stem cell (NSC) lines and one fetal brain-derived NSC line to study inherent cell type heterogeneity at proliferating neural stem cell stage and uncovered predisposed presence of neurogenic and gliogenic progenitors. We observed heterogeneity in neurogenic progenitors that differed between the iPS cell-derived NSC lines and the fetal-derived NSC line, and we also observed differences in spontaneous differentiation potential for inhibitory and excitatory neurons between the iPS cell-derived NSC lines and the fetal-derived NSC line. In addition, using a recently published glia patterning protocol we enriched for gliogenic progenitors and generated glial cells from an iPS cell-derived NSC line.

中文翻译：

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对源自人类iPSC的神经干细胞的单细胞研究揭示了具有神经原性和胶质生成潜能的独特祖细胞群。

我们在几种人类诱导多能干（iPS）细胞源性神经干细胞（NSC）系和一条胎儿脑源性NSC细胞系上使用了单细胞RNA测序（seq），以研究在神经干细胞增殖阶段和内源性细胞中固有的细胞类型异质性。揭示神经源性和胶质生成性祖细胞的易感存在。我们观察到iPS细胞来源的NSC系和胎儿来源的NSC系之间神经源性祖细胞的异质性，并且我们观察到iPS细胞来源的NSC系与胎儿神经源性祖细胞之间抑制性和兴奋性神经元的自发分化潜能也存在差异。派生的NSC线。此外，使用最近发布的神经胶质细胞成像方案，我们丰富了胶质生成祖细胞，并从iPS细胞衍生的NSC系生成了神经胶质细胞。