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Morphology and molecular phylogeny of Oxytricha seokmoensis sp. nov. (Hypotrichia: Oxytrichidae), with notes on its morphogenesis.
European Journal of Protistology ( IF 1.9 ) Pub Date : 2019-09-27 , DOI: 10.1016/j.ejop.2019.125641
Kang-San Kim 1 , Gi-Sik Min 2
Affiliation  

A new hypotrichous ciliate, Oxytricha seokmoensis sp. nov., was discovered in a soil from a forest in South Korea and described based on the observations of living and stained specimens. In addition, phylogenetic analyses were performed using the small subunit ribosomal RNA (18S rRNA) gene sequence. Morphologically, the new species is similar to the O. granulifera-complex in terms of ciliary structure and arrangement of cortical granules, but dorsal kineties 3 and 4 (not completely separated vs. separated) and macronuclear nodules in the cyst (separated vs. fused) differ. Oxytricha seokmoensis is most similar to O. pulvillus, but can be distinguished by the number of adoral membranelles (30–40 vs. 23–27), contractile vacuole (present vs. absent), number of left (27–37 vs. 17–25) and right (27–35 vs. 18–23) marginal cirri, and lepidosomes on the cyst surface (present vs. absent). In a phylogenetic tree, O. seokmoensis is distinctly separated from the O. granulifera clade, but is sister to the Paroxytricha clade. In addition, O. seokmoensis and P. longigranulosa have the smallest genetic difference (d = 0.015, 23 of 1579 nt difference). This close relationship is supported by incomplete dorsal kinety 3 fragmentation and separated macronuclear nodules in resting cysts.



中文翻译:

土木香氧化菌的形态和分子系统发育。十一月 (Hypotrichia:Oxytrichidae),并对其形态进行了注释。

一种新的纤毛纤毛虫,Oxytricha seokmoensis sp。nov。,是在韩国森林的土壤中发现的,并根据活体和染色标本的观察进行了描述。此外,使用小亚基核糖体RNA(18S rRNA)基因序列进行了系统发育分析。从形态上讲,新物种在纤毛结构和皮质颗粒排列方面类似于颗粒状O. granulifera,但是在囊肿中背侧运动3和4(未完全分离或分离)和大核结节(分离或融合)。 )不同。Seokmoensis的OxytrichaO. pulvillus最相似,但可以通过动脉膜的数目(30–40对23–27),收缩液泡(当前与不存在),左侧(27–37对17–25)和右侧(27–35)来区分vs. 18–23)边缘性卷囊,以及囊肿表面的脂质体(存在或不存在)。在系统发育树中,seokmoensis O.与粒果O.进化枝明显分开,但是Paroxytricha进化枝的姐妹。此外,O。seokmoensisP. longigranulosa具有最小的遗传差异(d = 0.015,1579 nt差异中的23个)。背侧运动因子3断裂不完全和静止性囊肿中分离的大核结节支持了这种密切关系。

更新日期:2019-09-27
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