Brucella serostatus was evaluated in 3189 muskoxen sampled between 1989 and 2016 from various locations of the Canadian Arctic archipelago and mainland, near the communities of Sachs Harbour and Ulukhaktok, Northwest Territories, and Cambridge Bay and Kugluktuk, Nunavut. Brucella antibodies were found only in muskoxen sampled around Cambridge Bay, both on southern Victoria Island and on the adjacent mainland (Kent Peninsula). Consistent with participatory epidemiology data documented from local harvesters describing increased Brucella-like syndromes (swollen joints and lameness) and a decreased proportion of juveniles, the apparent Brucella seroprevalence in the sampled muskoxen of the Cambridge Bay area increased from 0.9% (95% CI 0.3–2.1) in the period of 1989–2001 to 5.6% (95% CI 3.3–8.9) in 2010–2016. The zoonotic bacteria Brucella suis biovar 4 was also cultured from tissues of muskoxen sampled on Victoria Island near Ulukhaktok in 1996 (n = 1) and Cambridge Bay in 1998, 2014, and 2016 (n = 3). Overall, our data demonstrate that B. suis biovar 4 is found in muskoxen that are harvested for food and by guided hunts on Victoria Island and Kent Peninsula, adding an important public health dimension to this study. Robust participatory epidemiology data on muskox health and diseases greatly enhanced the interpretation of our Cambridge Bay data and, combined with the serological and microbiological data, provide compelling evidence that the prevalence of B. suis biovar 4 has increased in this area since the late 1990s. This study enhances the available knowledge on Brucella exposure and infection in muskoxen and provides an example of how scientific knowledge and local knowledge can work together to better understand disease status in wildlife.

中文翻译：

---

1989-2016年，加拿大西部北极地区马斯科肯州布鲁切拉的跨学科研究方法。

对1989年至2016年间从加拿大北极群岛和大陆各地，西北地区萨克斯港和乌鲁卡克托克社区以及剑桥湾和努纳武特的库格鲁克图克社区附近的3189份麝香中提取的布鲁氏菌进行了评估。布鲁氏菌抗体仅在维多利亚岛南部和邻近大陆（肯特半岛）的剑桥湾附近的麝香中发现。与当地收割者记录的参与性流行病学数据一致，该数据描述了布鲁氏菌样综合症（关节肿胀和la行）增加，少年比例降低，明显的布鲁氏菌病剑桥湾地区的麝香中的血清阳性率从1989-2001年期间的0.9％（95％CI 0.3-2.1）增加到2010-2016年的5.6％（95％CI 3.3-8.9）。还从1996年（n  = 1）的乌鲁卡克托（Ulukhaktok）附近的维多利亚岛和1998、2014和2016（n  = 3）的剑桥湾（Cambridge Bay）采样的麝香糖的组织中培养了人畜共患的布鲁氏菌4型细菌。总体而言，我们的数据表明，猪双歧杆菌在muskoxen中发现了biovar 4，其被捕捞后用作食物，并通过在维多利亚岛和肯特半岛的狩猎进行了狩猎，为这项研究增加了重要的公共卫生方面。关于麝香健康和疾病的健壮的参与性流行病学数据大大增强了我们对剑桥湾数据的解释，并与血清学和微生物学数据相结合，提供了令人信服的证据，证明自1990年代后期以来，该地区的B. suis biovar 4患病率有所增加。这项研究增强了关于麝香中布鲁氏菌暴露和感染的现有知识，并提供了科学知识和当地知识如何协同工作以更好地了解野生动物疾病状况的一个例子。