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Progress towards a methodology for high throughput 3D reconstruction of soot nanoparticles via electron tomography
Journal of Microscopy ( IF 2 ) Pub Date : 2018-01-16 , DOI: 10.1111/jmi.12680
E Haffner-Staton 1 , A LA Rocca 1 , M W Fay 1, 2
Affiliation  

The aim of this work is to make progress towards the development of 3D reconstruction as a legitimate alternative to traditional 2D characterization of soot. Time constraints are the greatest opposition to its implementation, as currently reconstruction of a single soot particle takes around 5–6 h to complete. As such, the accuracy and detail gains are currently insufficient to challenge 2D characterization of a representative sample (e.g. 200 particles). This work is a consideration of the optimization of the steps included within the computational reconstruction and manual segmentation of soot particles. Our optimal process reduced the time required by over 70% in comparison to a typical procedure, whilst producing models with no appreciable decrease in quality.

中文翻译:

通过电子断层扫描对烟尘纳米粒子进行高通量 3D 重建的方法取得进展

这项工作的目的是在 3D 重建的发展方面取得进展,作为传统 2D 烟尘表征的合法替代方案。时间限制是其实施的最大阻力,因为目前单个烟尘粒子的重建需要大约 5-6 小时才能完成。因此,精度和细节增益目前不足以挑战代表性样本(例如 200 个粒子)的 2D 表征。这项工作是对烟尘粒子的计算重建和手动分割中包含的步骤的优化的考虑。与典型程序相比,我们的最佳流程将所需时间减少了 70% 以上,同时生产的模型质量没有明显下降。
更新日期:2018-01-16
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