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Phylogenetic Analysis of the Complete rRNA Gene Sequence of Nosema sp. SE Isolated from the Beet Armyworm Spodoptera exigua.
Journal of Parasitology ( IF 1.3 ) Pub Date : 2019-11-16
Dongxu Xing 1, 2 , Qingrong Li 1, 2 , Jiehua Zhang 3 , Yang Xiao 1, 2 , Weinan Chen 1, 2 , Qiong Yang 1, 2
Affiliation  

The microsporidium Nosema sp. SE is a pathogen that infects the beet armyworm Spodoptera exigua. The complete sequence of its 4,302-base pair (bp) ribosomal ribonucleic acid (rRNA) gene region was obtained by polymerase chain reaction amplification and sequencing. The rRNA organization of Nosema sp. SE was 5'-large subunit (LSU) rRNA-internal transcribed spacer-small subunit (SSU) rRNA-intergenic spacer-5S-3', which corresponded to the pattern of Nosema bombycis. Phylogenetic analysis based on LSU rRNA and SSU rRNA both indicated that the parasite had a close relationship with other true Nosema species, confirming that Nosema sp. SE belongs to true Nosema group of the genus Nosema.

中文翻译:

Nosema sp。的完整rRNA基因序列的系统发育分析。SE与甜菜夜蛾Spodoptera exigua隔离。

微孢子虫Nosema sp。SE是一种感染甜菜夜蛾夜蛾Spodoptera exigua的病原体。通过聚合酶链反应扩增和测序获得其4302个碱基对(bp)的核糖体核糖核酸(rRNA)基因区域的完整序列。Nosema sp。的rRNA组织。SE是5'-大亚基(LSU)rRNA-内部转录间隔子-小亚基(SSU)rRNA-基因间隔子-5S-3',其对应于Nosema bombycis的模式。基于LSU rRNA和SSU rRNA的系统发育分析均表明寄生虫与其他真正的Nosema物种密切相关,证实Nosema sp。SE属于Nosema属的真正Nosema组。
更新日期:2019-11-01
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