Acellular small-caliber tissue-engineered vascular grafts (SCTEVGs) have low patency rate due to complications including thrombosis and intimal hyperplasia. Rapid endothelialization, antithrombosis and antiproliferation approaches are suitable for dispelling these complications. Nevertheless, common antithrombosis and antiproliferation techniques are usually incompatible with rapid endothelialization on vascular grafts. To overcome these obstacles, we developed nanofibrous polyurethane scaffolds loaded with resveratrol drug, which is a natural compound extracted from plants and shows multifaceted effects in cardiovascular protection. It was found that the tensile strength and Young's modulus in modified scaffolds were significantly increased by resveratrol loading into membranes. The tensile strengths and breaking strains of resveratrol-loaded scaffolds were close to that of native vessels. The resveratrol release profile from the nanofibrous scaffolds occurred in a sustained manner. The anti-thrombogenicity of resveratrol-loaded nanofibers increased compared to polyurethane alone, with the result that prolonged human blood clotting time and lower hemolysis were detected on these scaffolds. The viability of human umbilical vein endothelial cells and smooth muscle cells on resveratrol-loaded scaffolds was evaluated. Our findings demonstrated that resveratrol-loaded nanofibers resulted in not only appropriate antithrombotic properties, but the formation of a monolayer of endothelial cells on the scaffold surface and lower smooth muscle cell growth. These resveratrol-loaded nanofibers are suggested as potential scaffolds for SCTEVGs.

中文翻译：

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负载白藜芦醇的聚氨酯纳米纤维支架：内皮细胞和平滑肌细胞的活力。

由于包括血栓形成和内膜增生在内的并发症，无细胞小口径组织工程化血管移植物（SCTEVGs）的通畅率低。快速的内皮化，抗血栓形成和抗增殖方法适合消除这些并发症。然而，普通的抗血栓形成和抗增殖技术通常与血管移植物的快速内皮化不兼容。为了克服这些障碍，我们开发了负载白藜芦醇药物的纳米纤维聚氨酯支架，白藜芦醇是一种从植物中提取的天然化合物，在心血管保护方面显示出多方面的作用。发现通过白藜芦醇加载到膜中，改性支架的抗张强度和杨氏模量显着增加。负载白藜芦醇的支架的抗张强度和断裂应变接近天然血管。从纳米纤维支架中的白藜芦醇释放曲线持续发生。与单独的聚氨酯相比，负载白藜芦醇的纳米纤维的抗血栓形成性增强，结果是在这些支架上检测到延长的人类血液凝固时间和较低的溶血作用。评估了人脐静脉内皮细胞和平滑肌细胞在白藜芦醇负载的支架上的生存能力。我们的研究结果表明，负载白藜芦醇的纳米纤维不仅导致适当的抗血栓形成特性，而且在支架表面上形成单层内皮细胞​​并降低平滑肌细胞的生长。