BACKGROUND Bats are an important ecological group within ecosystems. The rabies virus is a Lyssavirus, and haematophagous bats are the principal reservoir; however, the virus has also been detected in non-haematophagous bats. The objective was to determine the rabies virus in non-haematophagous bats in the Colombian Caribbean region. METHODS In 2017, a cross-sectional study was carried out with a base-risk sampling in twelve geographic zones of the Colombian Caribbean area that included the main ecosystems of two departments. 286 bats were captured, which were euthanized with a pharmacological treatment following the ethical protocols of animal experimentation. The taxonomic identification was done with dichotomous keys. The necropsy was carried out at the capture site, and brain samples were kept in liquid nitrogen. The extraction of the RNA was carried out from the frozen brains with Trizol™; a fragment of 914 bp of the glycoprotein G of the rabies virus was amplified with RT-PCR. The amplicons were sequenced with the Sanger method. RESULTS Twenty-three genera of bats were identified, and, in two frugivorous, Artibeus lituratus and Artibeus planirostris, amplicons were obtained and sequenced as the rabies virus. CONCLUSIONS This is the first evidence of natural infection of the rabies virus in frugivorous bats in the Colombian Caribbean area; this result is important for the surveillance and control of rabies.

中文翻译：

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哥伦比亚加勒比地区的节食蝙蝠是狂犬病病毒的宿主。

背景技术蝙蝠是生态系统中重要的生态群体。狂犬病毒是一种狂犬病病毒，食血蝙蝠是主要的病毒库。但是，在非食血蝙蝠中也检测到了这种病毒。目的是确定哥伦比亚加勒比地区非食血蝙蝠中的狂犬病毒。方法2017年，在哥伦比亚加勒比海地区的十二个地理区域（包括两个部门的主要生态系统）进行了基础风险抽样的横断面研究。捕获了286只蝙蝠，并按照动物实验的道德规范对它们进行了药理处理以使其安乐死。分类识别使用二分键进行。在捕获部位进行尸检，并将脑样本保存在液氮中。RNA的提取是用Trizol™从冷冻的大脑中进行的；用RT-PCR扩增狂犬病病毒的糖蛋白G的914bp的片段。用Sanger方法对扩增子进行测序。结果鉴定出23种蝙蝠，并在两个食食性的Artibeus lituratus和Planibstristris中获得了扩增子并测序为狂犬病毒。结论这是在哥伦比亚加勒比海地区的食肉蝙蝠中狂犬病毒自然感染的第一个证据。这个结果对于狂犬病的监测和控制很重要。在两个节食的Artibeus lituratus和Artibeus planirostris中，获得了扩增子并测序为狂犬病毒。结论这是在哥伦比亚加勒比海地区的食肉蝙蝠中狂犬病毒自然感染的第一个证据。这个结果对于狂犬病的监测和控制很重要。在两个食食性的Artibeus lituratus和Artibeus planirostris中，获得了扩增子并测序为狂犬病毒。结论这是在哥伦比亚加勒比海地区的食肉蝙蝠中狂犬病毒自然感染的第一个证据。这个结果对于狂犬病的监测和控制很重要。