Bombyx mori monocarboxylate transporter 9 (BmMCT9) is involved in the transport of uric acid in silkworm integument.,Genes to Cells

当前位置： X-MOL 学术 › Genes Cells › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Bombyx mori monocarboxylate transporter 9 (BmMCT9) is involved in the transport of uric acid in silkworm integument.
Genes to Cells ( IF 1.3 ) Pub Date : 2019-12-13 , DOI: 10.1111/gtc.12734
Lingyan Wang _{1,

2} , Yaru Yin ₁ , Kun Wang ₁ , Jun Cao ₁ , Tingcai Cheng ₁ , Chun Liu ₁ , Yan Zhang _{1,

2} , Yong Zhu ₁

Affiliation

In Bombyx mori, there are more than 30 mutant strains exhibiting the translucent larval skin resulting from a decrease in the uric acid content in epidermal cell. Of these, the Chinese translucent (oc) mutant presents a moderately translucent larval skin. Previously, we narrowed the region linked to the oc phenotype to approximately 234 kb by positional cloning, and found that BmMCT9 was severely suppressed in the mutant. Here, we analyzed the mutation and potential molecular function of BmMCT9. Sequence analysis showed that a 2,624-bp fragment of BmMCT9 promoter region was replaced by a 22-bp sequence in the mutant. Luciferase reporter gene assay confirmed that BmMCT9 promoter activity in the mutant was significantly lower than that in the wild type. Knockdown of BmMCT9 caused a translucent phenotype in the first-instar silkworm larvae. Immunoblotting analysis showed that BmMCT9 expression was severely reduced in the mutant than in the wild type, and immunofluorescence showed that BmMCT9 existed mainly within the cytoplasm of epidermal cells. Together, our results suggest that reduced levels of BmMCT9 were responsible for the translucent phenotype of oc mutant, and that BmMCT9 might function in intracellular vesicles facing the cytoplasm including urate granules in silkworm integument.

更新日期：2019-11-01

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11