Background Neuroticism is a core personality trait and a major risk factor for several mental and physical diseases, particularly in females, who score higher on neuroticism than men, on average. However, a better understanding of the expression profiles of proteins in the circulating blood of different neurotic female populations may help elucidate the intrinsic mechanism of neurotic personality and aid prevention strategies on mental and physical diseases associated with neuroticism. Methods In our study, female subjects were screened for inclusion by the Eysenck Personality Questionnaire (EPQ), Beck Depression Inventory (BDI), Beck Anxiety Inventory (BAI) scales and routine physical examination. Subjects who passed the examination and volunteered to participate were grouped by neuroticism using EPQ scores (0 and 1 = low neuroticism group; > 5 = high neuroticism group). Proteins in serum samples of the two neuroticism groups were identified using isobaric tags for relative and absolute quantification (iTRAQ) technology. Results A total of 410 proteins exhibited significant differences between high and low neuroticism, 236 proteins were significantly upregulated and 174 proteins were significantly downregulated. Combine the results of GO and KEGG enrichment analysis of differences proteins between high and low neuroticism with the PPI network, it could be observed that the Alpha-synuclein (SNCA), ATP7A protein (ATP7A), Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (GNG2), cyclin-dependent kinase 6 (CDK6), myeloperoxidase (MPO), azurocidin (AZU1), Histone H2B type 1-H (HIST1H2BH), Integrin alpha-M (ITGAM) and Matrix metalloproteinase-9 (MMP9) might participate in the intrinsic mechanism of neuroticism by regulating response to catecholamine stimulus, catecholamine metabolic process, limbic system development and transcriptional misregulation in cancer pathway. Conclusions Our study revealed the characteristics of the neurotic personality proteome, which might be intrinsic mechanism of the neurotic population.

中文翻译：

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基于 iTRAQ 的定量蛋白质组学分析为神经质的分子机制提供了见解。

背景 神经质是一种核心人格特征，也是几种精神和身体疾病的主要危险因素，尤其是女性，她们的神经质平均得分高于男性。然而，更好地了解不同神经质女性人群循环血液中蛋白质的表达谱可能有助于阐明神经质人格的内在机制，并有助于预防与神经质相关的精神和身体疾病的策略。方法 在我们的研究中，女性受试者通过艾森克人格问卷（EPQ）、贝克抑郁量表（BDI）、贝克焦虑量表（BAI）量表和常规体格检查进行筛选。通过考试并自愿参加的受试者使用 EPQ 分数按神经质分组（0 和 1 = 低神经质组；> 5 = 高度神经质组）。使用等压标签进行相对和绝对定量 (iTRAQ) 技术鉴定了两个神经质组的血清样本中的蛋白质。结果共有410个蛋白质在高低神经质之间表现出显着差异，236个蛋白质显着上调，174个蛋白质显着下调。将高低神经质差异蛋白GO和KEGG富集分析结果与PPI网络相结合，可以观察到α-突触核蛋白（SNCA）、ATP7A蛋白（ATP7A）、鸟嘌呤核苷酸结合蛋白G（I） /G(S)/G(O) 亚基 gamma-2 (GNG2)、细胞周期蛋白依赖性激酶 6 (CDK6)、髓过氧化物酶 (MPO)、azurocidin (AZU1)、Histone H2B type 1-H (HIST1H2BH)、整合素α-M（ITGAM）和基质金属蛋白酶9（MMP9）可能通过调节对儿茶酚胺刺激的反应、儿茶酚胺代谢过程、边缘系统发育和癌症通路中的转录失调参与神经质的内在机制。结论 我们的研究揭示了神经症人格蛋白质组的特征，这可能是神经症人群的内在机制。