A fluorescent fullerene nanoparticle (NP)-based lateral flow immunochromatographic assay (LFIA) was developed for the rapid and quantitative detection of C-reactive protein (CRP) in serum. The polyclonal CRP-antibody-conjugated fullerene NPs were simply prepared by 1-ethyl-3-(3-dimethyllaminopropyl)-carbodiimide hydrochloride coupling after carboxylation of fluorescent fullerene NPs. By applying the CRP-antibody-conjugated fullerene NPs to a lateral flow test strip, quantitative analysis of CRP in serum was possible at a concentration range of 0.1–10 ng/ml within 15 min. We anticipate that this novel fluorescent fullerene NP-based LFIA can be useful for the rapid and accurate sensing of biological and chemical species, contributing to the disease diagnosis and prognosis, environmental monitoring, and food safety.

中文翻译：

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基于荧光富勒烯纳米粒子的横向流免疫色谱分析法，用于快速定量检测C反应蛋白。

开发了一种基于荧光富勒烯纳米颗粒（NP）的侧向流免疫色谱分析（LFIA），用于快速定量检测血清中的C反应蛋白（CRP）。通过将荧光富勒烯NP羧化后，通过1-乙基-3-（3-二甲基氨基丙基）-碳二亚胺盐酸盐盐酸盐偶联，简单地制备了多克隆CRP-抗体缀合的富勒烯NP。通过将CRP抗体偶联的富勒烯NPs应用于侧流试纸条，可以在15分钟内以0.1–10 ng / ml的浓度范围对血清中CRP进行定量分析。我们预计，这种新颖的基于荧光富勒烯NP的LFIA可以用于快速，准确地感测生物和化学物种，有助于疾病的诊断和预后，环境监测以及食品安全。