Altered metabolism is one of the defining features of cancer. Since the discovery of the Warburg effect in 1924, research into the metabolic aspects of cancer has been reinvigorated over the past decade. Metabolomics is an invaluable tool for gaining insights into numerous biochemical processes including those related to cancer metabolism and metabolic aspects of other diseases. The combination of untargeted and targeted metabolomics approaches has greatly facilitated the discovery of many cancer biomarkers with prognostic potential. Using mass spectrometry-based stable isotope-resolved metabolomics (SIRM) with isotopic labeling, a powerful tool used in pathway analysis, researchers have discovered novel cancer metabolic pathways and metabolic targets for therapeutic application. Metabolomics technologies provide invaluable metabolic insights reflecting cancer progression in coordination with genomics and proteomics aspects. The systematic study of metabolite levels in the metabolome and their dynamics within a biological organism has been, in recent years, applied across a wide range of fields. Metabolomics technologies have been applied to both early clinical trials and pre-clinical research in several essential aspects of human health. This chapter will give an overview of metabolomics technologies and their application in the discovery of novel pathways using isotopic labeled and non-labeled metabolomics.

中文翻译：

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代谢组学技术在癌症预后和治疗中的应用。

新陈代谢改变是癌症的定义特征之一。自 1924 年发现 Warburg 效应以来，在过去十年中，对癌症代谢方面的研究重新焕发了活力。代谢组学是深入了解众多生化过程的宝贵工具，包括与癌症代谢和其他疾病代谢方面相关的生化过程。非靶向和靶向代谢组学方法的结合极大地促进了许多具有预后潜力的癌症生物标志物的发现。使用基于质谱的稳定同位素解析代谢组学 (SIRM) 和同位素标记（一种用于途径分析的强大工具），研究人员发现了新的癌症代谢途径和用于治疗应用的代谢靶点。代谢组学技术提供了宝贵的代谢见解，反映了与基因组学和蛋白质组学方面协调的癌症进展。近年来，对生物体内代谢组中代谢物水平及其动力学的系统研究已应用于广泛的领域。代谢组学技术已应用于人类健康几个重要方面的早期临床试验和临床前研究。本章将概述代谢组学技术及其在使用同位素标记和非标记代谢组学发现新途径中的应用。应用于广泛的领域。代谢组学技术已应用于人类健康几个重要方面的早期临床试验和临床前研究。本章将概述代谢组学技术及其在使用同位素标记和非标记代谢组学发现新途径中的应用。应用于广泛的领域。代谢组学技术已应用于人类健康几个重要方面的早期临床试验和临床前研究。本章将概述代谢组学技术及其在使用同位素标记和非标记代谢组学发现新途径中的应用。