Foodborne viruses are a global threat to food safety. Real-time reverse transcription polymerase chain reaction (RT-PCR) is the most commonly used method to detect viral RNA in food. Armored RNA (AR) prepared using the MS2 phage system is a successful positive control for detecting foodborne viruses and is an important quality control process when using real-time RT-PCR. In this study, we report a novel technology for preparing AR using bacteriophage Qβ and compare its stability with AR prepared using the MS2 phage system for packaging norovirus detection target RNA. AR could be successfully and efficiently produced using the developed bacteriophage Qβ system. Two types of AR–AR-QNoV prepared using the Qβ system and AR-MNoV prepared using the MS2 system—were stored at different temperatures for different durations. After incubating at − 20 °C for 360 days, the copy numbers of AR-QNoV and AR-MNoV decreased by 8.9% and 35.9%, respectively. After incubating at 4 °C for 60 days, the copy numbers of AR-QNoV and AR-MNoV decreased by 12.0% and 38.9%, respectively. After incubating at 45 °C, the copy numbers of AR-QNoV decreased by 71.8% after 5 days, whereas those of AR-MNoV decreased by 92.9% after only 4 days. After 5 days, AR-MNoV could not be detected using real-time RT-PCR. There was a significant difference in copy numbers decrease rate between AR-QNoV and AR-MNoV at three different temperatures (P < 0.05 ). Therefore, AR prepared using the new bacteriophage Qβ system is more stable than the traditional AR, making the developed strategy a good candidate for AR preparation and quality control.

中文翻译：

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使用噬菌体Qβ的新型铠装RNA技术的开发和评估。

食源性病毒是对食品安全的全球威胁。实时逆转录聚合酶链反应（RT-PCR）是检测食品中病毒RNA的最常用方法。使用MS2噬菌体系统制备的铠装RNA（AR）是检测食源性病毒的成功阳性对照，并且在使用实时RT-PCR时是重要的质量控制过程。在这项研究中，我们报告了一种使用噬菌体Qβ制备AR的新技术，并将其稳定性与使用MS2噬菌体系统制备的用于包装诺如病毒检测靶RNA的AR进行了比较。使用开发的噬菌体Qβ系统可以成功，有效地生产AR。使用Qβ系统制备的两种AR–AR-QNoV和使用MS2系统制备的AR-MNoV –在不同温度下存储了不同的持续时间。在− 20°C下孵育360天后，AR-QNoV和AR-MNoV的拷贝数分别下降了8.9％和35.9％。在4°C下孵育60天后，AR-QNoV和AR-MNoV的拷贝数分别下降了12.0％和38.9％。在45°C孵育后，AR-QNoV的拷贝数在5天后下降了71.8％，而AR-MNoV的拷贝数在仅4天后下降了92.9％。5天后，无法使用实时RT-PCR检测到AR-MNoV。在三种不同温度下，AR-QNoV和AR-MNoV的拷贝数减少率存在显着差异（5天后，AR-QNoV的拷贝数下降了71.8％，而仅4天后，AR-MNoV的拷贝数下降了92.9％。5天后，无法使用实时RT-PCR检测到AR-MNoV。在三种不同温度下，AR-QNoV和AR-MNoV的拷贝数减少率存在显着差异（5天后，AR-QNoV的拷贝数下降了71.8％，而仅4天后，AR-MNoV的拷贝数下降了92.9％。5天后，无法使用实时RT-PCR检测到AR-MNoV。在三种不同温度下，AR-QNoV和AR-MNoV的拷贝数减少率存在显着差异（P  ＜0.05）。因此，使用新的噬菌体Qβ系统制备的AR比传统的AR更稳定，从而使开发的策略成为AR制备和质量控制的良好选择。