Tonic immobility (TI) is an effective anti-predator strategy. However, long immobility status on the ground increases the risk of being eaten by predators, and thus insects must rouse themselves when appropriate stimulation is provided. Here, the strength of vibration causing arousal from the state of TI was examined in strains artificially selected for longer duration of TI (L-strains: long sleeper) in a beetle. We provided different strengths of vibration stimuli to the long sleepers in Tribolium castaneum. Although immobilized beetles were never awakened by the stimuli from 0.01 to 0.12 mm in amplitude, almost of the beetles were aroused from immobilized status by the stimulus at 0.21 mm. There was a difference in sensitivity of individuals when the stimuli of 0.14 mm and 0.18 mm were provided. F2 individuals were also bred by crossing experiments of the strains selected for shorter and longer duration of TI. The arousal sensitivity to vibration was well separated in the F2 individuals. A positive relationship was observed between the duration of TI and the vibration amplitude, suggesting that immobilized beetles are difficult to arouse from a deep sleep, while light sleepers are easily aroused by even small vibrations. The results indicate a genetic basis for sensitivity to arousal from TI.

中文翻译：

---

由振动刺激引起的强直停止。

补品固定（TI）是一种有效的反捕食者策略。然而，长期处于地面的静止状态增加了被掠食者吞噬的风险，因此，如果提供适当的刺激，昆虫必须唤醒自己。在此，在甲虫中人工选择的TI持续时间较长的菌株（L菌株：长卧铺）中检测了从TI状态引起的振动强度。我们为Tri藜的长枕提供了不同强度的振动刺激。尽管固定的甲虫从未被振幅从0.01到0.12 mm的刺激唤醒，但几乎所有的甲虫都被0.21 mm的刺激从固定状态唤醒。当提供0.14mm和0.18mm的刺激时，个体的敏感性存在差异。F2个体也通过交叉选育用于TI持续时间较短和较长的菌株进行繁殖。F2个体对振动的唤醒敏感度很好地分开了。TI的持续时间与振动幅度之间存在正相关关系，这表明固定的甲虫很难从深度睡眠中唤醒，而轻度睡眠者即使很小的振动也容易引起。结果表明TI对唤醒的敏感性具有遗传基础。轻型卧铺即使很小的振动也很容易引起。结果表明TI对唤醒的敏感性具有遗传基础。轻型卧铺即使很小的振动也很容易引起。结果表明TI对唤醒的敏感性具有遗传基础。