Abstract

Nowadays, medicines from plant sources play a vital role in healthcare management. Chrysin, a plant flavonoid, possesses a wide range of pharmacological activities. The aim of present investigation was to evaluate the safety of chrysin by determining toxicity after acute and sub-chronic oral administration in rats. Acute oral toxicity (AOT) and sub-chronic oral toxicity studies of chrysin were carried out according to OECD 425 and OCED 408 in Sprague Dawley rats. In AOT, oral administration of chrysin (5000 mg/kg) showed 40% mortality. In the sub-chronic toxicity study, daily oral administration of chrysin (1000 mg/kg) showed significantly decreased body weight whereas liver weight was increased significantly in male rats. A significant alteration in the hematology (RBC, MCH, MCHC, TLC, lymphocytes, and neutrophil) and blood chemistry (albumin, bilirubin, ALT, AST, creatinine, and GGT) were found in chrysin (1000 mg/kg) treated rats which were either limited to one sex or lacked dose-response or were within the normal laboratory ranges. There was a significant increase in hepatic and renal oxido-nitrosative stress in chrysin (1000 mg/kg) treated rats. There was no significant change in electrocardiographic (except heart rate), hemodynamic, the left ventricular function, and lung function test. Renal and hepatic histological aberrations were induced in chrysin (1000 mg/kg) treated rats. In conclusion results of the present investigation determined the LD₅₀ value of chrysin to be 4350 mg/kg whereas NOAEL and LOAEL of chrysin was found to be 500 and 1000 mg/kg, respectively for both the sexes.

摘要

如今，植物来源的药物在医疗保健管理中发挥着至关重要的作用。白杨素是一种植物类黄酮，具有广泛的药理活性。本研究的目的是通过测定大鼠急性和亚慢性口服给药后的毒性来评估白杨素的安全性。根据 OECD 425 和 OCED 408 在 Sprague Dawley 大鼠中进行了白杨素的急性经口毒性 (AOT) 和亚慢性经口毒性研究。在 AOT 中，口服白杨素 (5000 mg/kg) 的死亡率为 40%。在亚慢性毒性研究中，每日口服白杨素（1000 mg/kg）显示雄性大鼠体重显着降低，而肝脏重量显着增加。血液学（RBC、MCH、MCHC、TLC、淋巴细胞和中性粒细胞）和血液化学（白蛋白、在白杨素 (1000 mg/kg) 处理的大鼠中发现了胆红素、ALT、AST、肌酐和 GGT)，这些大鼠要么局限于一种性别，要么缺乏剂量反应，要么在正常的实验室范围内。白杨素 (1000 mg/kg) 处理的大鼠肝脏和肾脏氧化亚硝化应激显着增加。心电图（心率除外）、血流动力学、左心室功能、肺功能检查无明显变化。在白杨素 (1000 mg/kg) 处理的大鼠中诱发肾和肝组织学异常。总之，本调查的结果确定了 LD 白杨素 (1000 mg/kg) 处理的大鼠肝脏和肾脏氧化亚硝化应激显着增加。心电图（心率除外）、血流动力学、左心室功能、肺功能检查无明显变化。在白杨素 (1000 mg/kg) 处理的大鼠中诱发肾和肝组织学异常。总之，本调查的结果确定了 LD 白杨素 (1000 mg/kg) 处理的大鼠肝脏和肾脏氧化亚硝化应激显着增加。心电图（心率除外）、血流动力学、左心室功能、肺功能检查无明显变化。在白杨素 (1000 mg/kg) 处理的大鼠中诱发肾和肝组织学异常。总之，本调查的结果确定了 LD_{白杨素的50}值为 4350 毫克/公斤，而白杨素的 NOAEL 和 LOAEL 对两性分别为 500 和 1000 毫克/公斤。