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Approaches for measuring the dynamics of RNA-protein interactions.
WIREs RNA ( IF 6.4 ) Pub Date : 2019-08-20 , DOI: 10.1002/wrna.1565
Donny D Licatalosi 1 , Xuan Ye 1 , Eckhard Jankowsky 1
Affiliation  

RNA-protein interactions are pivotal for the regulation of gene expression from bacteria to human. RNA-protein interactions are dynamic; they change over biologically relevant timescales. Understanding the regulation of gene expression at the RNA level therefore requires knowledge of the dynamics of RNA-protein interactions. Here, we discuss the main experimental approaches to measure dynamic aspects of RNA-protein interactions. We cover techniques that assess dynamics of cellular RNA-protein interactions that accompany biological processes over timescales of hours or longer and techniques measuring the kinetic dynamics of RNA-protein interactions in vitro. This article is categorized under: RNA Interactions with Proteins and Other Molecules > Protein-RNA Interactions: Functional Implications RNA Interactions with Proteins and Other Molecules > Protein-RNA Recognition RNA Interactions with Proteins and Other Molecules > RNA-Protein Complexes RNA Evolution and Genomics > Ribonomics.

中文翻译:


测量 RNA-蛋白质相互作用动态的方法。



RNA-蛋白质相互作用对于从细菌到人类的基因表达调节至关重要。 RNA-蛋白质相互作用是动态的;它们在生物学相关的时间尺度上发生变化。因此,了解 RNA 水平上基因表达的调控需要了解 RNA-蛋白质相互作用的动力学。在这里,我们讨论测量 RNA-蛋白质相互作用动态方面的主要实验方法。我们涵盖了评估伴随数小时或更长时间的生物过程的细胞 RNA-蛋白质相互作用动力学的技术,以及测量体外 RNA-蛋白质相互作用动力学的技术。本文分类为:RNA 与蛋白质和其他分子的相互作用 > 蛋白质-RNA 相互作用:功能含义 RNA 与蛋白质和其他分子的相互作用 > 蛋白质-RNA 识别 RNA 与蛋白质和其他分子的相互作用 > RNA-蛋白质复合物 RNA 进化和基因组学 >核糖组学。
更新日期:2019-11-01
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