Single amino acids and small endogenous peptides play important roles in maintaining a properly functioning organism. These molecules are however currently only routinely identified in targeted approaches. In a small proof-of-concept mass spectrometry experiment, we found that by combining isobaric tags and peptidomics, and by targeting singly charged molecules, we were able to identify a significant amount of single amino acids and small endogenous peptides using a basic mass-based identification approach. While there is still room for improvement, our simple test indicates that a limited amount of extra work when setting up the mass spectrometry experiment could potentially lead to a wealth of additional information.

中文翻译：

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通过结合同量异位标记和肽组学检测单个氨基酸和小肽

单个氨基酸和小的内源性肽在维持正常运作的生物体方面发挥着重要作用。然而，这些分子目前仅在靶向方法中常规鉴定。在一个小型的概念验证质谱实验中，我们发现通过结合同量异位标记和肽组学，并通过靶向单电荷分子，我们能够使用基本质量鉴定大量的单个氨基酸和小的内源性肽。基于识别的方法。虽然仍有改进的空间，但我们的简单测试表明，在设置质谱实验时进行有限的额外工作可能会产生大量额外信息。