Many top-down proteomics experiments focus on identifying and localizing PTMs and other potential sources of “mass shift” on a known protein sequence. A simple application to match ion masses and facilitate the iterative hypothesis testing of PTM presence and location would assist with the data analysis in these experiments. ProSight Lite is a free software tool for matching a single candidate sequence against a set of mass spectrometric observations. Fixed or variable modifications, including both PTMs and a select number of glycosylations, can be applied to the amino acid sequence. The application reports multiple scores and a matching fragment list. Fragmentation maps can be exported for publication in either portable network graphic (PNG) or scalable vector graphic (SVG) format. ProSight Lite can be freely downloaded from http://prosightlite.northwestern.edu, installs and updates from the web, and requires Windows 7 or a higher version.

中文翻译：

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ProSight Lite：图形软件，用于分析自上而下的质谱数据。

许多自上而下的蛋白质组学实验着重于识别和定位PTM和已知蛋白质序列上“质量转移”的其他潜在来源。匹配离子质量并促进PTM存在和位置的迭代假设测试的简单应用将有助于这些实验中的数据分析。ProSight Lite是一个免费软件工具，用于将单个候选序列与一组质谱观测值进行匹配。固定的或可变的修饰，包括PTM和选定数目的糖基化，都可以应用于氨基酸序列。该应用程序报告多个分数和一个匹配的片段列表。碎片图可以导出为以便携式网络图形（PNG）或可伸缩矢量图形（SVG）格式发布。可以从http：// prosightlite免费下载ProSight Lite。