We study the metaproteome of the GF/F‐prefiltered fraction of a microbial community from Shantou coast summer surface waters using a shotgun proteomic approach. Spectra attributed to the marine Roseobacter clade (MRC), the oligotrophic marine Gammaproteobacteria (OMG) group and Flavobacteria dominated in the microbial community, accounting for 21.0%, 23.2% and 12.7% of all of the detected spectra, respectively, whereas the SAR 92 clade accounted for 50% of the OMG group. The abundance of TonB‐dependent receptors (TBDRs) was detected and the majority of TBDRs were attributed to the OMG, whereas a large number of ABC transporters matched to the MRC, which suggests niche separation in the microbial community. Expression of proteorhodopsin and RagB/SusD from Flavobacteria facilitates their attachment and growth on algal‐derived organic matter. Taurine and glycine betaine appear to be an important source of carbon and nitrogen for the Rhodobacteraceae and SAR11 cluster. The detection of carbon monoxide dehydrogenase, formate dehydrogenase, O‐acetylhomoserine sulfhydrylase and sulfur oxidation protein from the MRC demonstrated that members of the MRC play important roles in coastal ocean biogeochemical cycles. This study provides the first insight into functional processes occurring in microbial communities in coastal waters in the South China Sea.

中文翻译：

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元蛋白质组学揭示了南海北部一个生产性沿海系统中的主要微生物作用因子及其生物地球化学功能。

我们使用a弹枪蛋白质组学方法研究了汕头沿海夏季地表水微生物群落的GF / F预滤部分的元蛋白质组。光谱归因于海洋R oseobacter进化枝（MRC），海洋贫营养性伽马蛋白菌（OMG）组和Flavobacteria在微生物群落中占主导地位，分别占所有检测光谱的21.0％，23.2％和12.7％，而SAR 92进化枝占OMG组的50％。检测到大量的TonB依赖受体（TBDR），大多数TBDR归因于OMG，而大量ABC转运蛋白与MRC相匹配，这表明微生物群落中的利基分离。黄杆菌中的蛋白视紫红质和RagB / SusD的表达促进了它们在藻类有机物上的附着和生长。牛磺酸和甘氨酸甜菜碱似乎是红细菌科的重要碳和氮源和SAR11集群。从MRC中检测到一氧化碳脱氢酶，甲酸脱氢酶，O-乙酰高丝氨酸巯基化酶和硫氧化蛋白表明，MRC的成员在沿海海洋生物地球化学循环中起着重要作用。这项研究提供了对南海沿海水域微生物群落中发生的功能过程的初步了解。